extract TCGA barcodes and sample_type information from uuids
1
0
Entering edit mode
gv ▴ 40
@gv-6516
Last seen 19 months ago
United States

Hi,

I have a list of fileuuids obtained from TCGA portal. I am trying to use GenomicDataCommons package to extract sample type(whether normal or tumor) and TCGA barcodes associated with input fileuuids. From the manual I can get to this

resp = cases() %>% filter(~ project.project_id=='TCGA-LUAD') %>%
  GenomicDataCommons::select(c(default_fields(cases()),'samples.sample_type')) %>%
  response_all()

Can someone guide me how to extract TCGA barcode and sample type from list of uuids?

Thanks

GenomicDataCommons R • 2.2k views
ADD COMMENT
0
Entering edit mode
Kevin Blighe ★ 3.9k
@kevin
Last seen 6 days ago
Republic of Ireland

The answer can be found via Sean Davis: GenomicDataCommons Example: UUID to TCGA and TARGET Barcode Translation. Note that, once you infer the barcode from the UUID, you can then infer tumour|normal from the barcode.

Kevin

ADD COMMENT
0
Entering edit mode

HI Kevin, I used Sean's code and got the barcodes. How can I infer tumor|normal from them? For example if I have these barcodes:

TCGA-05-4249-01A
TCGA-05-4250-10A
TCGA-05-4382-01A
TCGA-05-4390-10A
TCGA-05-4395-01A

Thanks

ADD REPLY
0
Entering edit mode

Ah, for that, please take a look here: https://www.biostars.org/p/313063/

ADD REPLY
0
Entering edit mode

Hi Kevin Blighe Sean Davis , the link is really helpful to do the translation, and I found TCGAutils is helpful too.

But, I got a question. As below, "afe6dd5c-a32e-4ba8-a9b5-154b27a9513b" is the file_id. How about the prefix in the bam file or bai file? What id it is?

ls afe6dd5c-a32e-4ba8-a9b5-154b27a9513b
f689d486-2605-4110-946e-4b8831aa2f3d_gdc_realn_rehead.bai  f689d486-2605-4110-946e-4b8831aa2f3d_gdc_realn_rehead.bam  logs
ADD REPLY

Login before adding your answer.

Traffic: 444 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6