Concatenating different runs from the same pool of barcoded CyTOF samples
0
0
Entering edit mode
@mikhaelmanurung-17423
Last seen 21 months ago
Netherlands

Hello, I would like to ask a question about CyTOF. I have a set of files from multiple acquisition periods of the same pool of barcoded samples (14 clinical samples and 1 reference samples). We couldn't measure everything in one go because of, well, clogging and stuff. Here is the ridgeline plot of CD3 expression distribution across acquisitions.

https://ibb.co/X2NbjWc

I do not think there's a significant variation of marker expression distribution across acquisitions. What do you think? Would it be acceptable to just concatenate all samples and then debarcode?

Thanks in advance for your advice.

batch effect cytof • 605 views
ADD COMMENT

Login before adding your answer.

Traffic: 876 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6