Question: The proper way of analysisng FACS-data; Question off topis?
13.6 years ago by
Ulrik Stervbo • 30
Ulrik Stervbo • 30 wrote:
Hello All, I have a question regarding the proper way of analysing FACS-data. I realise that the question may be off topic and I apologise. If it is too far off, please advise me where else to go. I am relatively new to FACS measurements (though not new to programming). In the laboartory where I just started, they treat cell cultures in triplicates and measure for some parameter on the FACS, e.g. DNA fragmentation or exposure of phosphatidylserine. After FACS measurement, the percentage of cells with for instance fragmented DNA or exposed PS is found, and the mean form the triplicates is taken. Later bar-graphs with standard variations are created. As we collect at least 3 x 10.000 events, I somehow feel that collapsing them all onto basically just three measurements, and then ask if those three measurements are different from three other experiments may not be the best way to go. I would like to know if there is a significant difference after treatment with some compound, and also compare treatment times. Is there a better way than taking the means of triplicates? Any solution should produce bar-graphs with quantitative differences. Thanks in advance. Ulrik
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