Question: rflowcyt and prada questions
gravatar for Nolwenn LeMeur
13.5 years ago by
Nolwenn LeMeur140 wrote:
Hi Andras, Just a quick note to let you know that I've made some progress but I have not solved the problem yet. The problem comes from the $DATATYPE where in FCS2.0 the data are stored as integer (I) and in FCS3.0 as single float precision (F). I have also tried to read the files with prada and have the same problem. Nolwenn dr. Treszl Andr?s wrote: >Hi everyone, > >I am using the rflowcyt package to analyze data generated on a FACSAria >machine with the DiVa software. I collect data for 10 minutes after an >initial stimulation of the cells than I export the FCS 3.0 files to >rflowcyt. After reading in the data I noticed that the data on >channel "Time" do not correspond to the actual duration of the measurement. >Namely, when I collected data for 10 minutes (=600 sec), my time- scale in >rflowcyt spans to 250 "units", instead of 600. (I am calculating a lot of >statistics, thus the proper time-resolution would be very important.) >However, if I try to analyze the same data with another software (e. g. >FlowJo), the scale is correct, thus the data needed are within the file, >simply I am not able to extract it. What should I do? How can I get the >proper time-resolution? > >My second question: I have never been able to read in my FCS3.0 file from >the DiVa software via the prada package. Does prada recognize the DiVa- >derived FCS3.0 files? If not, do you have an extension for it? > >Your help is greatly appreciated, > >best > >Andras > >Andras Treszl MD PhD >First Department of Pediatrics, Semmelweis University, Budapest, Hungary >B?kay u. 53 >H-1083 Budapest, Hungary >treszl at >Tel.: 36/1/334-3186 >Fax.: 36/1/313-8212 > >_______________________________________________ >Bioconductor mailing list >Bioconductor at > >Search the archives: > >
prada rflowcyt • 514 views
ADD COMMENTlink written 13.5 years ago by Nolwenn LeMeur140
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