Hi John, "john bermingham" <johnber at="" campus.ie=""> writes: > Hello Ihave been having trouble downloading the bioconductor > software. Ihave followed the instructions as on your website but Im > not having any success. In the command window I type > source("http://www.bioconductor.org/biocLite.R")and I get the > following message: Error in file(file, "r", encoding = encoding) : > unable to open connection In addition: Warning message: unable to > connect to 'www.bioconductor.org' on port 80. Could you please tell > me what Im doing wrong and how to fix it. My computers operating > system is Windows 2000. Thank you It sounds like your system is having trouble connecting to the internet and talking to the Bioconductor website. Is your system behind a web proxy firewall that requires authentication? Your network administrator will be able to tell you. There is information about how to deal with web proxies in the help page for download.file. + seth