EDASeq: is it possible to normalize within lanes for more than one covariate?
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abf ▴ 30
@abf-14661
Last seen 2 days ago
United States

Is it appropriate to normalize within lanes for each covariate in sequence, for example:

dataWithin <- withinLaneNormalization(data,"gc", which="full")
dataWithin <- withinLaneNormalization(dataWithin,"length", which="full")
dataNorm <- betweenLaneNormalization(dataWithin, which="full")

If this is appropriate, are there any recommendations for method selection or order of operations?

EDASeq normalization GC length RNASeq • 186 views
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