> Date: Fri, 16 Jun 2006 09:07:52 -0700 (PDT) > From: yuhong ning <yhning at="" yahoo.com=""> > Subject: [BioC] log2 ratio as limma input > To: bioconductor at stat.math.ethz.ch > Content-Type: text/plain; charset=iso-8859-1 > > Hi, > > I am trying to use limma to analyze Mass Spec data for > differentially expressed proteins from wild type cell > and mutant cells. The input data I got from scientists > is the intensity ratio of Heavy-isotope/light-isotope > peptide. I want to read it into MAList, but seems the > ratio data only correspondent to M, I have no way to > obtain the "A" data. What should I do? > > > Thanks a lot. > > yuhong Dear Yuhong, All the limma commands you might want to use accept simple matrices as input, so I can't think of any reason why you would need to read you data into an MAList object. Best wishes Gordon