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michael baron IAH-P
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@michael-baron-iah-p-1880
Last seen 9.6 years ago
Hi all,
I trawled a bit back through the archives and couldn't see an answer
to this, so perhaps someone can help.
Limma uses a Printer Class to identify which print group a spot
belongs to. This data object includes the number of grids, their
arrangement and how many rows and columns of spots their are in each
grid. This is easy to add to an RGList manually. The package does not
seem to need a specific columns of info relating each spot to a
particular grid, so I am guessing that there is an assumption that the
functions are expecting the data to be arranged in a particular way.
Is this so? I am analysing some data that is arranged in what I
suspect is a non-standard way (all the spots in the first row across
the ARRAY first, then the second row of the array, and so on). Is
limma expecting the data to be 1st row of 1st grid, then 2nd row of
1st grid, and so on? If not, could someone tell me what it is
expecting, or perhaps just that I am totally wrong and limma is
working out the grid-spot linkage in some other way?
Thanks in advance,
Michael
*******************************************************
Michael D. Baron Ph.D.
Group Leader, Morbillivirus-Host Interactions
Institute for Animal Health
Ash Road,
Pirbright,
Surrey GU24 0NF
U.K.