Question: Limma: different numbers of duplicated spots
gravatar for Gordon Smyth
12.0 years ago by
Gordon Smyth37k
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Gordon Smyth37k wrote:
Dear Jeremy, Personally, I'd treat all the genes as duplicated twice. In this approach, the small group of special genes which are actually duplicated 20 times would each be treated as 10 different genes. Best wishes Gordon > Date: Wed, 4 Apr 2007 14:51:13 -0700 > From: "Jeremy Davis-Turak" <jeremydt at=""""> > Subject: [BioC] Limma: different numbers of duplicated spots > To: bioconductor at > Message-ID: > <378b225b0704041451h72a7fbb3hc206614aec7cdc27 at> > Content-Type: text/plain > > Hi BioC list, > > I am analyzing some new Agilent 4x44 C. Elegans arrays, and as our previous > Agilent celegans arrays, there are 120 genes that are printed many (> 10) > times. However, now on each array everything is duplicated: Those 120 spots > are printed 20 times (not 10), and all others are printed twice (and one > spot is printed 4 probably was meant to be 2 different genes). > As far as I can tell, the duplicated spots are randomly spaced. I would > like to use duplicateCorrelation on the normalized data, sorted by gene > name, as described previously on the list: > > 0ea8e/ > > My only problem now is the spots that are replicated 20 times. In the past > I haven't dealt with them using very stringent statistics, since it was only > 120 spots that I was dealing with (and maybe 1 gene in the group was > differentially expressed). Now however, since all 20K spots are duplicated, > we need to take of the duplicates. Clearly duplicateCorrelation is the > simplest way to do this, but it won't work if we have 120 genes that are > printed 20 times. > > My question is: how do I deal with these gens? Could I just ignore those > 120 genes for the calculation of the consensus correlation? I read on this > list that small numbers of genes won't affect this calculation: 120 /20K is > less than 1% of the genes. > > If I do that, what would become of the 120 spots? Can I somehow apply the > same consensus correlation to them? > > What other solutions do people propose? > > > Thanks in advance for your time. > > Jeremy Davis-Turak
celegans • 362 views
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