Hi, I am trying to use limma read.maimages to load some customized datafiles. when I tried to specify column names for Rf and Gf, like: colums=("Cy5", "Cy3"), I got sytax error. Could anybody tell me how I can pass the list? Thanks! Leon [[alternate HTML version deleted]]

Dear Leon, At 10:54 AM 18/06/2003, leon ding wrote: >Hi, > >I am trying to use limma read.maimages to load some customized datafiles. >when I tried to specify column names for Rf and Gf, like: colums=("Cy5", >"Cy3"), I got sytax error. >Could anybody tell me how I can pass the list? The limma help documentation for 'read.maimages' says: columns: list with fields `Rf', `Gf', `Rb' and `Gb' giving the column names to be used for red and green foreground and background. So you need something like columns=list(Rf="Cy5",Gf="Cy3",Rb="Cy5.background",Gb="Cy3.backgrou nd") The names in double quotes are the column names in your file. The rest of the syntax is compulsory! Gordon >Thanks! > >Leon > > [[alternate HTML version deleted]]