Hi,
I am trying to use limma read.maimages to load some customized
datafiles.
when I tried to specify column names for Rf and Gf, like:
colums=("Cy5", "Cy3"), I got sytax error.
Could anybody tell me how I can pass the list?
Thanks!
Leon
[[alternate HTML version deleted]]
Dear Leon,
At 10:54 AM 18/06/2003, leon ding wrote:
>Hi,
>
>I am trying to use limma read.maimages to load some customized
datafiles.
>when I tried to specify column names for Rf and Gf, like:
colums=("Cy5",
>"Cy3"), I got sytax error.
>Could anybody tell me how I can pass the list?
The limma help documentation for 'read.maimages' says:
columns: list with fields `Rf', `Gf', `Rb' and `Gb' giving the
column
names to be used for red and green foreground and
background.
So you need something like
columns=list(Rf="Cy5",Gf="Cy3",Rb="Cy5.background",Gb="Cy3.backgrou
nd")
The names in double quotes are the column names in your file. The rest
of
the syntax is compulsory!
Gordon
>Thanks!
>
>Leon
>
> [[alternate HTML version deleted]]