2 Test Samples on 1 Array
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@elliot-harrison-2391
Last seen 9.6 years ago
Hi All, Not strictly Bioconductor question I'm afraid. Is there any reason why we couldn't hybridise 2 samples to one array (1 green, 1 Red) and then perform single colour analysis on both samples independently? It would be a custom agilent array using the probe set from their whole human genome array. Many Thanks Elliott This message has been scanned for viruses by BlackSpider...{{dropped:3}}
probe probe • 605 views
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@sean-davis-490
Last seen 12 weeks ago
United States
elliot harrison wrote: > Hi All, > > Not strictly Bioconductor question I'm afraid. Is there any reason why > we couldn't hybridise 2 samples to one array (1 green, 1 Red) and then > perform single colour analysis on both samples independently? It would > be a custom agilent array using the probe set from their whole human > genome array. People do this, yes. I do not know how much crosstalk between channels there is on modern arrays. You will want to think a little about normalization, also. See the limma user guide for some examples of one-channel analysis of two-color data. Sean
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