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Wolfgang Raffelsberger
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160
@wolfgang-raffelsberger-1805
Last seen 10.3 years ago
Dear list,
the question might seem trivial, I just couldn't find a function to
transform a simple data.frame with microarray log2-ratios into a
limma-type ot marray-type objects.
I have a data.frame containing (R/G-) log2-ratios for the each slide
in
a separate column and I 'd like to use some of the functions available
in limma or marray, in particular to run a lowess normalization. I
realized that I need either limma or marray specific (S4-) objects but
I
couldn't find a function for easily creating one of these. I guess
I'll
need either a class 'marrayNorm' object or an 'MAList', but I
couldn't find a specific function to do so.
Or is it necessary to create an exprSet first, to be able to transform
the data the to an MAList ?
Any suggestions ?
Just for clarification : I run R-2.6.0 with BioC 2.1
Thank's in advance,
Wolfgang
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Wolfgang Raffelsberger, PhD
Laboratoire de BioInformatique et G?nomique Int?gratives
CNRS UMR7104, IGBMC
1 rue Laurent Fries, 67404 Illkirch Strasbourg, France
Tel (+33) 388 65 3300 Fax (+33) 388 65 3276
wolfgang.raffelsberger at igbmc.u-strasbg.fr