RE: mva.pairs
1
0
Entering edit mode
@stephen-henderson-71
Last seen 7.0 years ago
OK try something like >Sel<-c(1,4,5,6) or >Sel<- 1:3 then >mva.pairs(exprs(eset[,Sel])) REM if you are using rma or similar to put in log.it=F (so the results are not logged again). e.g. >mva.pairs(exprs(eset[,Sel]), log.it=F) If there's a wrong un in there you'll see aline of skewed or highly spread scatters. -----Original Message----- From: Anna Gustafsson [mailto:annag@biotech.kth.se] Sent: Thursday, August 14, 2003 2:26 PM To: Stephen Henderson Subject: mva.pairs OK! Seems to generate plots. But how can I decide which chips to be compared to each other? I have 7 chips in this particular test experiment and want to be able to test each against all others more or less. Is it "doable" :)? Thanks for helping out! // Anna :o) At 14:14 2003-08-14 +0100, you wrote: >Try >>mva.pairs(exprs(eset)) > >-----Original Message----- >From: Anna Gustafsson [mailto:annag@biotech.kth.se] >Sent: Thursday, August 14, 2003 2:13 PM >To: bioconductor@stat.math.ethz.ch >Subject: [BioC] (no subject) > >Dear all, > >Unfortunately - more questions: >I wonder how to use mva.pairs to plot 2 replicate slides from an "eset" >object of background corrected, normalized, pm corrected and summarized data >(i.e rma output). > >I can only find a vignette describing plots from only normalized, non >summarized (16 individual expression measures / gene plotted) and I do not >understand how to transfer that information to work for my data in the >"eset" format..... :( > >With hopes on help! > >// Anna :o) > >********************************************************************* ****** * >*************** >Anna Gustafsson > >Royal Institute of Technology >AlbaNova University Center >Stockholm Center for Physics, Astronomy and Biotechnology >Department of Molecular Biotechnology >106 91 Stockholm, Sweden > >Phone (office): +46 8 553 783 41 >Fax: + 46 8 553 784 81 >Visiting adress: Roslagstullsbacken 21, Floor 3 >Delivery adress: Roslagsv?gen 30B > >_______________________________________________ >Bioconductor mailing list >Bioconductor@stat.math.ethz.ch >https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > >********************************************************************* * >This email and any files transmitted with it are confidential and >intended solely for the use of the individual or entity to whom they >are addressed. If you have received this email in error please notify >the system manager (wibr.mail@ucl.ac.uk). All files are scanned for viruses. >********************************************************************* * ********************************************************************** ****** *************** Anna Gustafsson Royal Institute of Technology AlbaNova University Center Stockholm Center for Physics, Astronomy and Biotechnology Department of Molecular Biotechnology 106 91 Stockholm, Sweden Phone (office): +46 8 553 783 41 Fax: + 46 8 553 784 81 Visiting adress: Roslagstullsbacken 21, Floor 3 Delivery adress: Roslagsv?gen 30B ********************************************************************** ****** *************** ********************************************************************** This email and any files transmitted with it are confidentia...{{dropped}}
• 810 views
ADD COMMENT
0
Entering edit mode
@stephen-henderson-71
Last seen 7.0 years ago
ps to make it quicker you may also wish to only sample some genes (not 22,000) Try >samp<- sample(1:22000, 1000, replace=F) then >mva.pairs(exprs(eset[samp,Sel]), log.it=F) -----Original Message----- From: Anna Gustafsson [mailto:annag@biotech.kth.se] Sent: Thursday, August 14, 2003 2:26 PM To: Stephen Henderson Subject: mva.pairs OK! Seems to generate plots. But how can I decide which chips to be compared to each other? I have 7 chips in this particular test experiment and want to be able to test each against all others more or less. Is it "doable" :)? Thanks for helping out! // Anna :o) At 14:14 2003-08-14 +0100, you wrote: >Try >>mva.pairs(exprs(eset)) > >-----Original Message----- >From: Anna Gustafsson [mailto:annag@biotech.kth.se] >Sent: Thursday, August 14, 2003 2:13 PM >To: bioconductor@stat.math.ethz.ch >Subject: [BioC] (no subject) > >Dear all, > >Unfortunately - more questions: >I wonder how to use mva.pairs to plot 2 replicate slides from an "eset" >object of background corrected, normalized, pm corrected and summarized data >(i.e rma output). > >I can only find a vignette describing plots from only normalized, non >summarized (16 individual expression measures / gene plotted) and I do not >understand how to transfer that information to work for my data in the >"eset" format..... :( > >With hopes on help! > >// Anna :o) > >********************************************************************* ****** * >*************** >Anna Gustafsson > >Royal Institute of Technology >AlbaNova University Center >Stockholm Center for Physics, Astronomy and Biotechnology >Department of Molecular Biotechnology >106 91 Stockholm, Sweden > >Phone (office): +46 8 553 783 41 >Fax: + 46 8 553 784 81 >Visiting adress: Roslagstullsbacken 21, Floor 3 >Delivery adress: Roslagsv?gen 30B > >_______________________________________________ >Bioconductor mailing list >Bioconductor@stat.math.ethz.ch >https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > >********************************************************************* * >This email and any files transmitted with it are confidential and >intended solely for the use of the individual or entity to whom they >are addressed. If you have received this email in error please notify >the system manager (wibr.mail@ucl.ac.uk). All files are scanned for viruses. >********************************************************************* * ********************************************************************** ****** *************** Anna Gustafsson Royal Institute of Technology AlbaNova University Center Stockholm Center for Physics, Astronomy and Biotechnology Department of Molecular Biotechnology 106 91 Stockholm, Sweden Phone (office): +46 8 553 783 41 Fax: + 46 8 553 784 81 Visiting adress: Roslagstullsbacken 21, Floor 3 Delivery adress: Roslagsv?gen 30B ********************************************************************** ****** *************** ********************************************************************** This email and any files transmitted with it are confidentia...{{dropped}}
ADD COMMENT

Login before adding your answer.

Traffic: 723 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6