Filtering Affymetrix data. (Newbie)
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Marcus ▴ 150
@marcus-410
Last seen 9.6 years ago
Hello all!! How do I get access to the individual probe intensities in my data object (which I get with the data<-ReadAffy() command) ? I know that I can get them with the mm() and pm() functions but if I would like to see,filter,remove or change the data in the data object, how is this accomplished? Essentially: How is the data object built up and how do you access it?? Another question: How and where is the background value stored/calculated?? /Marcus ********************************************************************** ********************* Marcus Gry Bj?rklund Royal Institute of Technology AlbaNova University Center Stockholm Center for Physics, Astronomy and Biotechnology Department of Molecular Biotechnology 106 91 Stockholm, Sweden Phone (office): +46 8 553 783 39 Fax: + 46 8 553 784 81 Visiting adress: Roslagstullsbacken 21, Floor 3 Delivery adress: Roslagsv?gen 30B
probe probe • 745 views
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@james-w-macdonald-5106
Last seen 29 minutes ago
United States
You access the data by using the pm() and mm() functions. For instance: pm(abatch)[1:1000] <- 200 will replace the first 1000 pm values with 200. HTH, Jim James W. MacDonald Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623 >>> Marcus <marcusb@biotech.kth.se> 08/27/03 03:59AM >>> Hello all!! How do I get access to the individual probe intensities in my data object (which I get with the data<-ReadAffy() command) ? I know that I can get them with the mm() and pm() functions but if I would like to see,filter,remove or change the data in the data object, how is this accomplished? Essentially: How is the data object built up and how do you access it?? Another question: How and where is the background value stored/calculated?? /Marcus ********************************************************************** ********************* Marcus Gry Bj?rklund Royal Institute of Technology AlbaNova University Center Stockholm Center for Physics, Astronomy and Biotechnology Department of Molecular Biotechnology 106 91 Stockholm, Sweden Phone (office): +46 8 553 783 39 Fax: + 46 8 553 784 81 Visiting adress: Roslagstullsbacken 21, Floor 3 Delivery adress: Roslagsv?gen 30B _______________________________________________ Bioconductor mailing list Bioconductor@stat.math.ethz.ch https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor
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and you can read the vignette. sections 4.1 and chapters 6 and 7 have some info that may help. -r On Wed, 27 Aug 2003, James MacDonald wrote: > You access the data by using the pm() and mm() functions. > > For instance: > > pm(abatch)[1:1000] <- 200 > > will replace the first 1000 pm values with 200. > > HTH, > > Jim > > > > James W. MacDonald > Affymetrix and cDNA Microarray Core > University of Michigan Cancer Center > 1500 E. Medical Center Drive > 7410 CCGC > Ann Arbor MI 48109 > 734-647-5623 > > >>> Marcus <marcusb@biotech.kth.se> 08/27/03 03:59AM >>> > Hello all!! > > How do I get access to the individual probe intensities in my data object > (which I get with the > data<-ReadAffy() command) ? > > I know that I can get them with the mm() and pm() functions but if I would > like to see,filter,remove or change the data in the data object, how is > this accomplished? > > Essentially: How is the data object built up and how do you access it?? > > Another question: How and where is the background value stored/calculated?? > > /Marcus > > ******************************************************************** *********************** > Marcus Gry Bj?rklund > > Royal Institute of Technology > AlbaNova University Center > Stockholm Center for Physics, Astronomy and Biotechnology > Department of Molecular Biotechnology > 106 91 Stockholm, Sweden > > Phone (office): +46 8 553 783 39 > Fax: + 46 8 553 784 81 > Visiting adress: Roslagstullsbacken 21, Floor 3 > Delivery adress: Roslagsv?gen 30B > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor >
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