Question: cellHTS2 - 2 channel batch analysis
0
gravatar for Steve Taylor
11.2 years ago by
Steve Taylor280
Steve Taylor280 wrote:
Hi, I am trying to set up a two channel analysis using cellHTS2. I have cell line X. X has control (C) and experiment (E) conditions. The screen uses 3 x 96 well plates and C and E have 2 replicates each, hence C and E have 6 plates each in total. It gets complicated because there is a repeat of the whole experiment (on a separate day) and the experimenter used a different plate configuration. Also 3 of the control plates were not run, so for the second experimental run C has 3 plates and E has 6 plates. I'd like to combine both experiments but wondered what is the best way? I read you can normalize for batch effects which looks like it should help but on the whole I am unsure how to configure this analysis. Thanks for any help, Steve ------------------------------------------------------------------ Medical Sciences Division Weatherall Institute of Molecular Medicine/Sir William Dunn School Oxford University
cellhts2 • 353 views
ADD COMMENTlink written 11.2 years ago by Steve Taylor280
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 277 users visited in the last hour