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Daniel Brewer
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@daniel-brewer-1791
Last seen 9.6 years ago
Hello,
I have three types of samples PC3M (the control cell line), Knockdown
(PC3M with an siRNA knockdown vector) and Scramble (PC3M with a vector
with a scrambled sequence). I have set up my design matrix like this:
design <- modelMatrix(targets,ref="PC3M")
As one of the comparisons I would like to compare the knockdown vs the
controls. How would one do this?
Is something like this correct?
contrast.matrix <- makeContrasts("Knockdown vs Controls"=(2*Knockdown
-
Scramble)/2)
Thanks
Dan
--
**************************************************************
Daniel Brewer, Ph.D.
Institute of Cancer Research
Molecular Carcinogenesis
Email: daniel.brewer at icr.ac.uk
**************************************************************
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