Question: Does the strand of a microarray probe matter?
11.0 years ago by
Cei Abreu-Goodger • 830
Cei Abreu-Goodger • 830 wrote:
Hello all, Related issues have arisen before, where the probe of a particular array platform was annotated to a gene on the opposite strand. But I was just asked if this even matters, or should it simply be considered a case of bad probe design. Does the protocol for different manufacturer's arrays always produce amplified product of both strands for the transcript to be measured? I could imagine that protocols that amplify based on poly-A tails would tend to produce an anti-sense biased amplification product (older Affy arrays?), whereas those based on random priming could produce products of both strands (and so the actual strand that is on the array becomes meaningless). Does someone know what is the case in particular for Illumina Beadarrays? Many thanks, Cei -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE.
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