Hi, I am using the current version of LIMMA (January 25, 2009) and am having the following error message from this script below: Error Message: Error in new("AnnotatedDataFrame", data = phenotypicdata) : trying to generate an object from a virtual class ("AnnotatedDataFrame") Problematic Script (Note this script was originally run successfully under the version of LIMMA current during the winter/spring of 2008, but is not working now): > setClass("AnnotatedDataFrame") [1] "AnnotatedDataFrame" > adf<-new("AnnotatedDataFrame",data=phenotypicdata) Here is my phenotypicdata: species time c0M1 c 0 c0M2 c 0 c0M3 c 0 c0M4 c 0 s0M1 s 0 s0M2 s 0 s0M3 s 0 c24M1 c 24 c24M2 c 24 c24M3 c 24 s24M1 s 24 s24M2 s 24 s24M3 s 24 s24M4 s 24 c48M1 c 48 c48M2 c 48 c48M3 c 48 s48M1 s 48 s48M2 s 48 s48M3 s 48 s48M4 s 48 c96M1 c 96 c96M2 c 96 c96M3 c 96 s96M1 s 96 s96M2 s 96 s96M3 s 96 s96M4 s 96 Thank-you Sally Goldes [[alternative HTML version deleted]]