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Steve Lauriault ▴ 120@steve-lauriault-3237
Last seen 7.3 years ago
I noticed the cloud function, and have tried to apply my flowFrame, but to no avail. Any hints on the annotation? The FCS file can be named MyData or ?/Data/FMO.fcs?, and I would like to include FSC-H, SSC-H, and FL1-H into cloud(). FSC-H and SSC-H would be in linear, and FL1-H in log (10^0 to 10^4) scale. I think it would be cool to add a fourth colour-based dimension to a cloud plot. Let's say FL2-H is on scale[0,1]. Any relative fluorescence intensity of 0 would correspond to a yellow event, and a relative fluorescence intensity of 1 would correspond to red. 0.5 would be orange. Any value ascending from zero would become more-red and less-yellow. The light wavelength (colour) of the ?dot? wold be directly related to the fluorescence intensity of the corresponding event. The user would have the option to have the fourth dimension enabled or disabled (in the case of colours representing offspring gates, this function would complicate things). It would also be cool to ?cycle-through? FCS file visualizations within a 4-D cloud-based GUI. Is there a function or package like this currently available in R? These are just some thoughts. I hope you guys don't mind. Now back to figuring out how to use lapply to create a new flowSet out of separate directories located in "/Data" (HowTo-flowCore 2.2.1). Thanks, Stevan ? ? ?