Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene Quantification homepage. The focus of this newsletter issue is: - qPCR 2009 Event => http://www.qPCR2009.net - we are proud to present 230 scientific contributions (86 talks & 144 posters) have a look on the preliminary online agenda => http ://online-AGENDA.qpcr2009.net/ - real-time PCR Cycler page updated => http://cyclers.gene- quantification.info/ - webinar page updated => http://webinar.gene-quantification.info/ - Online translation service of the Gene Quantification => http://translation.gene-quantification.info/ New qPCR workshop modules at the TATAA Biocenter Germany => http://tataa.gene-quantification.info/ European wide qPCR application workshops => register now ! => course program spring - summer 2009 => http://www.gene-quantification.de/bioeps-courses-spring- summer-2009.pdf ---------------------------------------------------------------------- ---------- real-time PCR Cycler On this page the most prominent real-time PCR cycler are described. In the cycler descriptions the specifications and the advantages of the displayed systems are shown. Which real-time platform meets your requirements best, depends on your research application. Some of the systems are designed for research with low capacities and others are for high-throughput applications, most in combination with pipetting robots. Most of them use solid-block for thermal cycling, other use hot- and cooled-air. Most differences are obviously in the application software, especially in the way of data analysis and how the derived crossing points or threshold levels are computed. Each of these systems employs either one of several general types of fluorescent probes for detection. There are also big differences how data are displayed. Some of the limitations of end-point detection in (RT-) PCR have been assuaged in real-time PCR systems, a number of which are now on the market. These systems offer many general technical advantages, including reduced probabilities of variability and contamination, as well as online monitoring and the lack of need for post reaction analyses. Further, some of these systems were developed with contemporary applications such as quantitative PCR, multiplexing, and high-throughput analysis in mind. Initial template levels can be calculated by analysing the shape of the curve or by determining when the signal rises above some threshold value. Several commercially available real-time PCR systems are overviewed on this page and/or summarized in the accompanying table. => http://cyclers.gene-quantification.info/ ---------------------------------------------------------------------- ---------- NEW reviews Real-Time PCR: Current Technology and Applications http://www.horizonpress.com/realtimepcr Editor: Julie Logan, Kirstin Edwards and Nick Saunders Applied and Functional Genomics, Health Protection Agency, London (2009) ISBN: 9781904455394 Publisher: Caister Academic Press Chapter 4 - Reference Gene Validation Software for Improved Normalization J. Vandesompele, M. Kubista and M. W. Pfaffl (2009) http://www.gene-quantification.de/Vandesompele-Kubista-Pfaffl-real- time-PCR-chapter-4.pdf Real-time PCR is the method of choice for expression analysis of a limited number of genes. The measured gene expression variation between subjects is the sum of the true biological variation and several confounding factors resulting in non-specific variation. The purpose of normalization is to remove the non-biological variation as much as possible. Several normalization strategies have been proposed, but the use of one or more reference genes is currently the preferred way of normalization. While these reference genes constitute the best possible normalizers, a major problem is that these genes have no constant expression under all experimental conditions. The experimenter therefore needs to carefully assess whether a certain reference gene is stably expressed in the experimental system under study. This is not trivial and represents a circular problem. Fortunately, several algorithms and freely available software have been developed to address this problem. This chapter aims to provide an overview of the different concepts. Chapter 5 - Data Analysis Software M. W. Pfaffl, J. Vandesompele and M. Kubista (2009) http://www.gene-quantification.de/Pfaffl-Kubista-Vandesompele-real- time-PCR-chapter-5.pdf Quantitative real-time RT-PCR (qRT-PCR) is widely and increasingly used in any kind of mRNA quantification, because of its high sensitivity, good reproducibility and wide dynamic quantification range. While qRT-PCR has a tremendous potential for analytical and quantitative applications, a comprehensive understanding of its underlying principles is important. Beside the classical RT-PCR parameters, e.g. primer design, RNA quality, RT and polymerase performances, the fidelity of the quantification process is highly dependent on a valid data analysis. This review will cover all aspects of data acquisition (trueness, reproducibility, and robustness), potentials in data modification and will focus particularly on relative quantification methods. Furthermore useful bioinformatical, biostatical as well as multi-dimensional expression software tools will be presented. ---------------------------------------------------------------------- ---------- Webinars in qPCR & RNAinterference & Molecular-Biology Here you find a huge summary of webinars in the field of qPCR & RNAinterference & Molecular-Biology => http://webinar.gene-quantification.info/ ---------------------------------------------------------------------- ---------- online translation Since October 2008 we provide an online translation service of the Gene Quantification pages to several languages. Please recognize this is an automatic and robotic based translation service, and therefore we can give NO guarantee about the generated content. It should help to understand the "rough" content of the Gene Quantification pages, but still the original is the ENGLISH version: http://translation.gene-quantification.info/ http://www.gene-quantification.asia http://chinese.gene-quantification.asia/ http://dutch.gene-quantification.info/ http://french.gene-quantification.info/ http://german.gene-quantification.info/ http://greek.gene-quantification.info/ http://italian.gene-quantification.info/ http://japanese.gene-quantification.asia/ http://korean.gene-quantification.asia/ http://portuguese.gene-quantification.info/ http://russian.gene-quantification.asia/ http://spanish.gene-quantification.info/ ---------------------------------------------------------------------- ---------- With the new qPCR INFO PORTAL and all the presented tools we will help you with to find the right information about qPCR and related topics in Molecular Biology in the literature and in the World Wide Web. => Papers / Protocols / Methods / Databases / Alets / Feeds / Books / Forums / E-mail / Directory http://infoportal.gene-quantification.info/ ---------------------------------------------------------------------- ---------- Upcoming Events World-wide academic and commercial qPCR Events http://events.gene-quantification.info/ Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars, qPCR Education Program, etc. Please submit your qPCR event here => events@gene- quantification.info ---------------------------------------------------------------------- ---------- qPCR 2009 EVENT - 9 - 13 March 2009 more news here => http://www.qPCR2009.net download event FLYER => http://www.bioeps.com/qpcr2009/qPCR-2009 -3rd-announcement.pdf We are proud to present 230 scientific contributions (86 talks & 144 posters) have a look on the preliminary online agenda => http ://online-AGENDA.qpcr2009.net/ It is a pleasure to announce the Nobel Prize Laureate Kary Mullis at the qPCR 2009 event in an own session “25th Anniversary of PCR” List of confirmed speakers => http://speakers.qpcr2009.net/ An industrial exhibition with the 30 world leading companies will be held during the qPCR Symposium March 9-11 => http://exhibition.qpcr2009.net/ Our sponsors => http://sponsors.qpcr2009.net/ Please register until 31st December to get the early registartion fee => http://registration.qpcr2009.net/ Keynote lectures The Pioneer in PCR Kary B. Mullis 1993 Nobel Prize in Chemistry - 25th Anniversary of PCR Stephen A. Bustin Professor of Molecular Science, Institute of Cell and Molecular Science, Queen Mary's School of Medicine and Dentistry, University of London, UK - A new qPCR assay for the detection of Clostridium difficile - MIQE- guidelines for publication of qPCR data Ken Livak Senior Scientific Fellow, Fluidigm Corporation,San Francisco, CA, US - Moving from qPCR Assays to qPCR Arrays. ---------------------------------------------------------------------- ---------- qPCR WORKSHOP BioEPS GmbH / TATAA Biocenter Germany - qPCR Application workshops At the TATAA Biocenter Germany we offer qPCR application workshops, a 3-day qPCR Core Module and a 2-day qPCR Biostatistics Module. All courses are held regularly in Göteborg, Sweden, in English and in Freising-Weihenstephan, Germany, in German and English, and in Prague, Czech Republic in English and Czech. Depending on the occasion the workshop language and the different prices may apply. Further customized workshops and specialized trainings will be held as well across Europe and world-wide. TATAA Biocenter Germany workshops are held in cooperation with BioEPS GmbH, located at the campus of the Technical University of Munich, in Freising-Weihenstephan, very close to the Munich Airport (MUC). For more information and registration, please see our web page: => http://TATAA.gene-quantification.info/ Course Occasions 2009: 3-day qPCR Core Module (Mon. - Wed.) 2-day BioStatistics Module (Thu. - Fri.) 3-day single-cell qPCR Module (Mon. - Wed.) 3-day microRNA Module (Mon. - Wed.) 20 - 22 April 2009 (E) NEW microRNA qPCR 27 - 29 April 2009 (E) NEW singel-cell qPCR Application Workshop 11 - 15 May 2009 (Deutsch) 3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics Module (Thu. - Fri.) 15 - 19 Juni 2009 (E) 3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics Module (Thu. - Fri.) 13 - 15 Juli 2009 (E) NEW microRNA qPCR 27 - 31 Juli 2009 (E) 3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics Module (Thu. - Fri.) => http://www.gene-quantification.de/bioeps-courses-spring- summer-2009.pdf ---------------------------------------------------------------------- ---------- Forward Please send the qPCR NEWS to further scientists and friends who are interested in qPCR ! Best regards, Michael W. Pfaffl responsible Editor of the Gene Quantification Pages http://www.gene-quantification.info ---------------------------------------------------------------------- ---------- If this newsletter is not displayed correctly by your email client, please use following link: http://qpcrnews.gene-quantification.info/ The qPCR NEWS and the Gene Quantification Pages are educational sites with the only purpose of facilitating access to qPCR related information on the internet. The qPCR NEWS and the Gene Quantification Pages are edited by Michael W. Pfaffl. Copyright © 2005 - 2009 All rights reserved. Any unauthorized use, reproduction, or transfer of this message or its contents, in any medium, is strictly prohibited. Disclaimer & Copyrights are displayed on the homepage www.gene-quantification.com The qPCR newsletter was end to bioconductor@stat.math.ethz.ch To subscribe or change your e-mail address in qPCR NEWS, and if you would like to receive future issues FREE of charge, please send an e-mail with the subject SUBSCRIBE to mailto:newsletter@gene- quantification.info?subject=SUBSCRIBE To unsubscribe from the qPCR NEWS, please send an e-mail with the subject UNSUBSCRIBE to mailto:newsletter@gene- quantification.info?subject=UNSUBSCRIBE_bioconductor@stat.math.ethz.ch [[alternative HTML version deleted]]