> eset = justRMA >  library(limma) >  targets = readTargets("target.txt") > targets >  lev <- c("SP","SN") >  f <- factor(targets$Targets, levels=lev) > design <- model.matrix(~0+f) >  colnames(design) <- lev >  fit <- lmFit( eset, design) Error in as.vector(x, mode) :  cannot coerce type 'closure' to vector of type 'any' this is the message i am getting, when i try to work with around 117 cel files. Target file and design seems to ok. but still i am getting error message. Please some one help regard this. Many Thanks Bharathi Connect with friends all over the world. Get Yahoo! India Messenger at http://in.messenger.yahoo.com/?wm=n/ [[alternative HTML version deleted]]

Hi Bharathi, Ayyarappan Bharathi wrote: >> eset = justRMA If this is really the code you are using, the problem is here. Instead of computing RMA, you are simply assigning the rma() function to another variable, 'eset'. What you want is eset <- justRMA() Best, Jim >> library(limma) >> targets = readTargets("target.txt") >> targets >> lev <- c("SP","SN") >> f <- factor(targets$Targets, levels=lev) >> design <- model.matrix(~0+f) >> colnames(design) <- lev >> fit <- lmFit( eset, design) > Error in as.vector(x, mode) : > cannot coerce type 'closure' to vector of type 'any' > > > this is the message i am getting, when i try to work with around 117 cel files. > Target file and design seems to ok. but still i am getting error message. > Please some one help regard this. > Many Thanks > Bharathi > > > Connect with friends all over the world. Get Yahoo! India Messenger at http://in.messenger.yahoo.com/?wm=n/ > [[alternative HTML version deleted]] > > > > -------------------------------------------------------------------- ---- > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Douglas Lab 5912 Buhl 1241 E. Catherine St. Ann Arbor MI 48109-5618 734-615-7826