Entering edit mode

John Antonydas Gaspar
▴
130

@john-antonydas-gaspar-3144
Last seen 8.1 years ago

Dear Sir/Madam,
I wish to do ANOVA p value computation with Bioconductor.
I have RMA normalized dataset in tab delimited text.
Data<-read.table("RMA-
normalized.txt",row.names=1,sep="\t",header=TRUE,dec
= ".",as.is =TRUE,na.strings = "NA",
colClasses = NA,check.names = FALSE,strip.white =
FALSE, blank.lines.skip = TRUE,
allowEscapes = FALSE, flush = FALSE,encoding =
"unknown")
dim(Data)
[1] 31528 51
17 * 3 = 51 There are 17 different cell line type samples with 3
biological replicates.
I wish to do ANOVA - p value calculation for finding out the
differentially expressed genes.
Kindly help me out to use the right Bioconductor package. Using
multtest
package I tried to do F stat but could not. When I try
the R console window goes off. Could be due to many number of
couloumns?.
Please help me out to find a solution.
Thanking you in advance,
With kind regards,
antony