Illumina: how to deal with locs, idat and tif files
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@jean-baptiste-poullet-3340
Last seen 9.6 years ago
Dear all, I would like to use the package "beadarray" on Illumina data. From Beadscan, I get files of the following formats: tif, locs, xml, one text file (Metrics.txt) and one sdf file. I managed to use Genomestudio and its genotyping module adding the bpm, csv and egt files (found on the illumina website, via ftp). Reading some tutorial on "beadarray" I noticed that 1 csv file per tif was required which I don't have. Moreover, the csv file I have does not show the same format as the one mentioned in this tutorial: the columns labels are IlmnID Name IlmnStrand SNP AddressA_ID AlleleA_ProbeSeq AddressB_ID AlleleB_ProbeSeq GenomeBuild Chr MapInfo Ploidy Species Source SourceVersion SourceStrand SourceSeq TopGenomicSeq BeadSetId CNV_Probe Intensity_Only Exp_Clusters instead of Index, Code, Gm, Red, x,y. It also seems that targets.txt is needed which I don't have either. Do you have any clue how to deal with these data given I don't have any of these txt or csv files? Thank you in advance. Jean-Baptiste
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