limma design question - technical replicates
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Georg Otto ▴ 510
@georg-otto-956
Last seen 9.6 years ago
Hi, I have a question about dealing with a design that is a mixture of technical and biological replicates. It's 4 two color-arrays with the dye-swap structure: 1,-1,1,-1 array 3 and 4 are technical replicates of the same biological source, so the biological replicate structure is: 1,2,3,3 This doesn't exactly fit the examples given in chapter 8.2 of the user's guide, maybe someone could give me a hint about how to deal with this design. Thanks a lot, Georg
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