Question: within array normalization for one color agilent array.??
9.3 years ago by
neeraj rana • 100
neeraj rana • 100 wrote:
hi, In the package Agi4x44PreProcess I took the expression value as *MeanSigna*l, then by the method *BGandNorm,* the background subtraction and the normalization between the array is done.But the array should be normalized within the array also first.This the script what is given in Agi4x44PreProcess for normalization.I didnt find any option for within the array normalization. > ddNORM = BGandNorm(dd, BGmethod = "half", NORMmethod = "quantile", + foreground = "MeanSignal", background = "BGMedianSignal", + offset = 50, makePLOTpre = FALSE, makePLOTpost = FALSE) please help me out how can we do the normalization within the array before between the array normalization for one color agilent array. thank you. [[alternative HTML version deleted]]
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