Please Could anybody tell me what is the problem? It does not happens in windows. I was revisiting the read.images code and it seems that the problem is related to some wt.fun identification or somethig. because it tryes to analize the code of the wt.fun function. Am I right? library(limma) + path=paste(base.path,"UCCOR/Investigacion/Osvaldo/Chile/Directa",sep ="") + targetfiles=readTargets("DirectaSinNormal_TargetFile.csv",path=path,se p=",") + signal.cols=list(R="F647 Median",Rb="B647 Median",G="F555 Median",Gb="B555 Median") + RG=read.maimages(paste(path,"/",targetfiles$FileName,sep=""), + source="genepix.median",quote = "\"'",columns=signal.cols,wt.fun = wtflags(0), + other.columns="Flags") Error in gsub("\\.", "\\\\.", x) : input string 1 is invalid in this locale Calls: read.maimages ... switch -> read.columns -> grep -> protectMetachar -> gsub > sessionInfo() R version 2.10.0 (2009-10-26) x86_64-unknown-linux-gnu locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 LC_PAPER=en_US.UTF-8 LC_NAME=en_US.UTF-8 [9] LC_ADDRESS=en_US.UTF-8 LC_TELEPHONE=en_US.UTF-8 LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=en_US.UTF-8 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] limma_3.2.1 SystemUtils_1.0 rkward_0.5.1 loaded via a namespace (and not attached): [1] tools_2.10.0 -- Elmer A. Fernández (Bioing. PhD) Investigador Asistente CONICET - Research Assistant CONICET Porf. Inteligencia Artificial -UCC - Prof. Artificial Intelligence @ UCC tel: +54-(0)351-4938000 int 145 Fax: +54-(0)351-4938081 web page : http://www.uccor.edu.ar/modelo.php?param=3.8.5.15 http://sites.google.com/site/biologicaldatamininggroup/Home/ mail address: Camino Alta Gracia Km 7.1/2- Córdoba-5000-Argentina [[alternative HTML version deleted]]