Entering edit mode
At 01:01 PM 31/12/2003, Simon Melov wrote:
>Gordon,
>when you assign zero weights to spots with flags, is this via the
wtfun?
>(i.e. as in the guide )
> > RG <- read.maimages(files,source="genepix",wt.fun=wtflags(0.1))
>
>or is there another way to specifically id certain types of flags in
limma
>(eg -50 vs -100)?
>Thanks
You can specify any weight function that you like, but you have to
define
it yourself. For example, if you wish to give zero weight to all flags
less
than -50 you could use
myfun <- function(x) as.numeric(x$Flags > -50.5)
RG <- read.maimages(files, source="genepix", wt.fun=myfun)
Gordon
>S.