Hi everyone. If I have the predicted target genes of miRNAs with no P values of difference expression, could I do the functional enrichment analysis of these target genes with your developed GOstats (R package)? Some say it could not do that but I found some do it. Here are scripts that someone used to do the functional enrichment analysis of a gene list with no P values of difference expression ( http://hi.baidu.com/ajiang12345/blog/item/84033cf5a70e152dbd310947.htm l). But I get the different result from DAVID. Please see it in the attachment file or please download from http://www.brsbox.com/filebox/down/fc/121c4cf4bc2658fe63c0b8f7d454abda . Could tell me what make the difference? And could you tell me how to select the value of categorySize? library(org.Hs.eg.db) library(GOstats) library(GO.db) geneIds <- c("10406", "10418", "11082", "1281", "1410", "157506", "167410", "1906" , "2029", "23091", "2625" , "2823" , "2877", "2993", "3039" , "3043", "3046", "3283", "3553", "4015", "4069", "4258", "4345", "4353", "4885", "4886", "5021", "5055", "5151", "5156", "5320", "5553", "55885", "56667", "5788" , "5999", "6005", "629", "6507", "653145", "6590", "673", "6876", "695", "7026", "7070", "7103", "7412", "760", "7738", "800", "828", "83890", "945", "963") univ <- Lkeys(org.Hs.egGO) param <- new("GOHyperGParams", geneIds = geneIds, universeGeneIds=univ, annotation="org.Hs.eg.db", ontology="BP") hyp <- hyperGTest(param) result<-summary(hyp,categorySize=2000) hist(pvalues(hyp), breaks=50, col="mistyrose") write.table(result,file="result-all-3-geneid-BP.txt",sep="\t") ---------------------------------------------------- The result I get is : GOBPID Pvalue OddsRatio ExpCount Count Size Term 1 GO:0032501 0.0001568145 3.021968 11.976486 24 3438 multicellular organismal process 2 GO:0032502 0.0012459810 2.626265 10.297409 20 2956 developmental process 3 GO:0007275 0.0051457709 2.457897 7.517527 15 2158 multicellular organismal development 4 GO:0007154 0.0061081457 2.187407 13.418681 22 3852 cell communication -- Zhidong -------------- next part -------------- Annotation Cluster 1 Enrichment Score: 3.6053856252516914 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0043627~response to estrogen stimulus 7 12.962962962962962 1.378612119569747E-6 1410,2625,760,5156,5021,3553,4886 47 105 13528 19.1886524822695 0.0014657690160471137 7.331532648784389E-4 0.002195364083090734 GOTERM_BP_FAT GO:0048545~response to steroid hormone stimulus 8 14.814814814814813 3.484745441921204E-6 1410,2625,760,5156,5021,3553,4886,4015 47 192 13528 11.99290780141844 0.003700910298128468 0.001235161762491277 0.005549178509367536 GOTERM_BP_FAT GO:0032355~response to estradiol stimulus 5 9.25925925925926 3.267552066770207E-5 1410,5156,5021,3553,4886 47 54 13528 26.65090622537431 0.03416988247807051 0.008654165748880338 0.05202182143972944 GOTERM_BP_FAT GO:0009725~response to hormone stimulus 8 14.814814814814813 2.1956417137320897E-4 1410,2625,760,5156,5021,3553,4886,4015 47 367 13528 6.274218795292481 0.20835476963210953 0.045653423523490666 0.3490751824817617 GOTERM_BP_FAT GO:0009719~response to endogenous stimulus 8 14.814814814814813 3.9940341889720306E-4 1410,2625,760,5156,5021,3553,4886,4015 47 405 13528 5.685526661413186 0.3462629853021123 0.046129910985666434 0.6341423786636047 GOTERM_BP_FAT GO:0010033~response to organic substance 10 18.51851851851852 6.143711138201849E-4 1410,1281,2625,760,5156,5021,3553,4886,629,4015 47 721 13528 3.9920913624693837 0.47998194756114987 0.057712363778680165 0.973889660724514 GOTERM_BP_FAT GO:0051240~positive regulation of multicellular organismal process 6 11.11111111111111 0.0013740139743678367 760,5021,3553,6507,4886,1906 47 244 13528 7.077781653296127 0.7684492391475862 0.09922238188647736 2.165771834172592 GOTERM_BP_FAT GO:0060341~regulation of cellular localization 5 9.25925925925926 0.009843658618997714 1410,5021,3553,4886,1906 47 248 13528 5.803019903912149 0.9999731580101143 0.1969049055873272 14.575146171946063 GOTERM_BP_FAT GO:0051046~regulation of secretion 4 7.4074074074074066 0.031127337352047094 5021,3553,4886,1906 47 202 13528 5.699599747208763 0.9999999999999976 0.35770605094996255 39.563051950229486 Annotation Cluster 2 Enrichment Score: 2.456691639484199 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0002683~negative regulation of immune system process 4 7.4074074074074066 0.0027953729402302985 1281,7070,2877,5788 47 83 13528 13.871315047423737 0.9491279528532574 0.13836375239097942 4.359859205012184 GOTERM_BP_FAT GO:0050777~negative regulation of immune response 3 5.555555555555555 0.0032286626237957457 1281,2877,5788 47 25 13528 34.53957446808511 0.967962708812792 0.14478509144469376 5.019481915819279 GOTERM_BP_FAT GO:0048585~negative regulation of response to stimulus 4 7.4074074074074066 0.004725662030567938 1281,2877,3553,5788 47 100 13528 11.513191489361702 0.9935263974929746 0.1416367967579798 7.265788187762134 Annotation Cluster 3 Enrichment Score: 1.9985392847515948 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0009617~response to bacterium 5 9.25925925925926 0.00410497989609644 5553,5320,2877,3553,4069 47 193 13528 7.4567302392239005 0.9874330870579516 0.14470623245084535 6.340541407642764 GOTERM_BP_FAT GO:0006952~defense response 8 14.814814814814813 0.004430763812887888 5553,2625,5320,4353,3553,629,5788,4069 47 615 13528 3.7441273136135615 0.9911275800631262 0.13726718885790923 6.827258730733088 GOTERM_BP_FAT GO:0042742~defense response to bacterium 3 5.555555555555555 0.05553829092965229 5553,5320,4069 47 112 13528 7.709726443768996 1.0 0.46225918731051696 59.74494536480752 Annotation Cluster 4 Enrichment Score: 1.8761057400705479 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0042060~wound healing 6 11.11111111111111 4.5629335808404373E-4 653145,1281,5156,3553,4015,5055 47 191 13528 9.041773420964686 0.38467637559628864 0.04740047867029662 0.724160644743066 GOTERM_BP_FAT GO:0030198~extracellular matrix organization 3 5.555555555555555 0.04867266146190952 1281,5156,4015 47 104 13528 8.302782324058919 1.0 0.4384589919647325 54.823431373163075 GOTERM_BP_FAT GO:0043062~extracellular structure organization 3 5.555555555555555 0.10596282743693812 1281,5156,4015 47 163 13528 5.297480746638819 1.0 0.6420575403614808 83.1981809967029 Annotation Cluster 5 Enrichment Score: 1.5689404654603825 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0032355~response to estradiol stimulus 5 9.25925925925926 3.267552066770207E-5 1410,5156,5021,3553,4886 47 54 13528 26.65090622537431 0.03416988247807051 0.008654165748880338 0.05202182143972944 GOTERM_BP_FAT GO:0007243~protein kinase cascade 8 14.814814814814813 2.3077972847647784E-4 695,1410,5999,673,5021,3553,1906,5788 47 370 13528 6.223346751006325 0.21774771032311646 0.04010335480012295 0.3668755973940119 GOTERM_BP_FAT GO:0000165~MAPKKK cascade 6 11.11111111111111 3.8455483734122593E-4 1410,5999,673,5021,3553,5788 47 184 13528 9.385753931544865 0.3358485316635216 0.04986920989542076 0.6106345390234802 GOTERM_BP_FAT GO:0051480~cytosolic calcium ion homeostasis 5 9.25925925925926 6.765802114709688E-4 5021,3553,4886,1906,5788 47 118 13528 12.196177425171296 0.5133085763079872 0.05824527512809041 1.0720051854555046 GOTERM_BP_FAT GO:0051240~positive regulation of multicellular organismal process 6 11.11111111111111 0.0013740139743678367 760,5021,3553,6507,4886,1906 47 244 13528 7.077781653296127 0.7684492391475862 0.09922238188647736 2.165771834172592 GOTERM_BP_FAT GO:0001568~blood vessel development 6 11.11111111111111 0.0013991087006320069 1281,7070,7026,3553,1906,4015 47 245 13528 7.048892748588797 0.7745583749666964 0.09454068608062627 2.2049151773433695 GOTERM_BP_FAT GO:0001944~vasculature development 6 11.11111111111111 0.0015568683718070442 1281,7070,7026,3553,1906,4015 47 251 13528 6.880393320335678 0.8094420192730993 0.09842534079377785 2.450656439676402 GOTERM_BP_FAT GO:0050870~positive regulation of T cell activation 4 7.4074074074074066 0.002174548048344717 7412,7070,3553,5788 47 76 13528 15.148936170212766 0.9013560546074315 0.1273749566393726 3.4072544389836423 GOTERM_BP_FAT GO:0003012~muscle system process 5 9.25925925925926 0.00249491605249053 1410,800,5021,3553,1906 47 168 13528 8.566362715298887 0.9299052715750058 0.13054602416202687 3.899937668778064 GOTERM_BP_FAT GO:0006469~negative regulation of protein kinase activity 4 7.4074074074074066 0.003194389410038875 7070,5999,3553,5788 47 87 13528 13.233553436047933 0.9667689471884284 0.14965073997167333 4.967461455567756 GOTERM_BP_FAT GO:0006874~cellular calcium ion homeostasis 5 9.25925925925926 0.0033950290184059147 5021,3553,4886,1906,5788 47 183 13528 7.864201836995698 0.9731759589616591 0.14557299652337385 5.271616661116363 GOTERM_BP_FAT GO:0046888~negative regulation of hormone secretion 3 5.555555555555555 0.003490170556399395 3553,4886,1906 47 26 13528 33.211129296235676 0.9757669296843273 0.1435861441323516 5.4155252824941265 GOTERM_BP_FAT GO:0033673~negative regulation of kinase activity 4 7.4074074074074066 0.003515474473020927 7070,5999,3553,5788 47 90 13528 12.792434988179668 0.9764128933182642 0.13919062769359403 5.453764822247131 GOTERM_BP_FAT GO:0055074~calcium ion homeostasis 5 9.25925925925926 0.0037385317809496567 5021,3553,4886,1906,5788 47 188 13528 7.65504753282028 0.9814122396557614 0.14210959570286352 5.790225079561118 GOTERM_BP_FAT GO:0051348~negative regulation of transferase activity 4 7.4074074074074066 0.004215311139247962 7070,5999,3553,5788 47 96 13528 11.99290780141844 0.9888305256772203 0.14357214536737806 6.505641197653778 GOTERM_BP_FAT GO:0006875~cellular metal ion homeostasis 5 9.25925925925926 0.004336158558686266 5021,3553,4886,1906,5788 47 196 13528 7.34259661311333 0.9901836363425812 0.14283383196952992 6.68616439343136 GOTERM_BP_FAT GO:0051251~positive regulation of lymphocyte activation 4 7.4074074074074066 0.004339572548517443 7412,7070,3553,5788 47 97 13528 11.86926957666155 0.9902193842413354 0.138663000455445 6.691259500878266 GOTERM_BP_FAT GO:0008217~regulation of blood pressure 4 7.4074074074074066 0.004725662030567938 5021,3043,4886,1906 47 100 13528 11.513191489361702 0.9935263974929746 0.1416367967579798 7.265788187762134 GOTERM_BP_FAT GO:0055065~metal ion homeostasis 5 9.25925925925926 0.005082296688643103 5021,3553,4886,1906,5788 47 205 13528 7.020238713025428 0.9955788340807771 0.14738870289773276 7.7935388060866195 GOTERM_BP_FAT GO:0002696~positive regulation of leukocyte activation 4 7.4074074074074066 0.0055587233688054495 7412,7070,3553,5788 47 106 13528 10.861501405058208 0.9973441255563928 0.15189385594326132 8.494167578085266 GOTERM_BP_FAT GO:0007204~elevation of cytosolic calcium ion concentration 4 7.4074074074074066 0.006160112237811897 5021,3553,1906,5788 47 110 13528 10.466537717601547 0.9986046786151767 0.1628003583141987 9.37144132014065 GOTERM_BP_FAT GO:0050867~positive regulation of cell activation 4 7.4074074074074066 0.0063163025619756685 7412,7070,3553,5788 47 111 13528 10.372244585010543 0.9988195507891418 0.1625698103195502 9.597990479213536 GOTERM_BP_FAT GO:0030005~cellular di-, tri-valent inorganic cation homeostasis 5 9.25925925925926 0.0072591063841199975 5021,3553,4886,1906,5788 47 227 13528 6.339863154934858 0.9995700575270345 0.18025799725260871 10.95426607178307 GOTERM_BP_FAT GO:0050863~regulation of T cell activation 4 7.4074074074074066 0.007303237139887878 7412,7070,3553,5788 47 117 13528 9.840334606292053 0.9995899201503985 0.17714803471243912 11.017280999251245 GOTERM_BP_FAT GO:0042102~positive regulation of T cell proliferation 3 5.555555555555555 0.007737575730714262 7412,3553,5788 47 39 13528 22.14075286415712 0.9997425787519741 0.18256215341645043 11.635253290850478 GOTERM_BP_FAT GO:0048584~positive regulation of response to stimulus 5 9.25925925925926 0.008303503117750438 7070,5320,3553,629,5788 47 236 13528 6.098088712585648 0.9998597118539159 0.18642829370718272 12.434415083022888 GOTERM_BP_FAT GO:0006873~cellular ion homeostasis 6 11.11111111111111 0.008503489992771469 5021,3553,4886,1906,5788,6005 47 374 13528 4.617590169530094 0.9998868053212374 0.18658115918802154 12.715198636865422 GOTERM_BP_FAT GO:0002684~positive regulation of immune system process 5 9.25925925925926 0.008548312051515175 7412,7070,3553,629,5788 47 238 13528 6.046844269622742 0.9998921211989658 0.18371272819494178 12.77801339155965 GOTERM_BP_FAT GO:0055066~di-, tri-valent inorganic cation homeostasis 5 9.25925925925926 0.008672478909057592 5021,3553,4886,1906,5788 47 239 13528 6.0215436659841535 0.9999055805190263 0.18247444089238318 12.95180276203225 GOTERM_BP_FAT GO:0055082~cellular chemical homeostasis 6 11.11111111111111 0.009073995132663944 5021,3553,4886,1906,5788,6005 47 380 13528 4.54468085106383 0.9999386429886982 0.18645779297609344 13.511563582804353 GOTERM_BP_FAT GO:0060341~regulation of cellular localization 5 9.25925925925926 0.009843658618997714 1410,5021,3553,4886,1906 47 248 13528 5.803019903912149 0.9999731580101143 0.1969049055873272 14.575146171946063 GOTERM_BP_FAT GO:0030003~cellular cation homeostasis 5 9.25925925925926 0.010679167914689685 5021,3553,4886,1906,5788 47 254 13528 5.665940693583515 0.9999890671861008 0.20795541028229958 15.715837448750547 GOTERM_BP_FAT GO:0051051~negative regulation of transport 4 7.4074074074074066 0.010790674263960924 1410,3553,4886,1906 47 135 13528 8.52828999211978 0.9999903027870506 0.20616007255274882 15.866988827190532 GOTERM_BP_FAT GO:0043405~regulation of MAP kinase activity 4 7.4074074074074066 0.012133625384494631 5999,3553,1906,5788 47 141 13528 8.165384035008298 0.9999977149906135 0.22484248719937894 17.66757142777258 GOTERM_BP_FAT GO:0050801~ion homeostasis 6 11.11111111111111 0.012209701588018072 5021,3553,4886,1906,5788,6005 47 409 13528 4.222441866514071 0.999997894763788 0.22226309354645846 17.76848298860574 GOTERM_BP_FAT GO:0050778~positive regulation of immune response 4 7.4074074074074066 0.013080101517659242 7070,3553,629,5788 47 145 13528 7.94013206162876 0.999999175961806 0.23227346112692293 18.914808409997175 GOTERM_BP_FAT GO:0051249~regulation of lymphocyte activation 4 7.4074074074074066 0.013817031240232466 7412,7070,3553,5788 47 148 13528 7.779183438757906 0.9999996277986609 0.23977817749857977 19.873626829836844 GOTERM_BP_FAT GO:0043086~negative regulation of catalytic activity 5 9.25925925925926 0.014304720620881712 7070,5999,3553,4886,5788 47 277 13528 5.19548352408019 0.9999997801087367 0.2432555649983149 20.50230484287382 GOTERM_BP_FAT GO:0051094~positive regulation of developmental process 5 9.25925925925926 0.01447802006172801 5320,760,5021,3553,5788 47 278 13528 5.176794734425226 0.999999817627704 0.24201610231653825 20.724588870757145 GOTERM_BP_FAT GO:0050671~positive regulation of lymphocyte proliferation 3 5.555555555555555 0.01498219460435864 7412,3553,5788 47 55 13528 15.69980657640232 0.9999998941959841 0.2455632339407936 21.367964864447085 GOTERM_BP_FAT GO:0051048~negative regulation of secretion 3 5.555555555555555 0.01498219460435864 3553,4886,1906 47 55 13528 15.69980657640232 0.9999998941959841 0.2455632339407936 21.367964864447085 GOTERM_BP_FAT GO:0006936~muscle contraction 4 7.4074074074074066 0.015097128450717525 1410,800,5021,1906 47 153 13528 7.524961757752747 0.999999906549528 0.2435101083073692 21.513945146759283 GOTERM_BP_FAT GO:0007267~cell-cell signaling 7 12.962962962962962 0.015381673037560162 945,5021,3553,6507,4258,4885,1906 47 600 13528 3.3580141843971627 0.9999999312833909 0.24387383536774043 21.8742601134708 GOTERM_BP_FAT GO:0032946~positive regulation of mononuclear cell proliferation 3 5.555555555555555 0.015503790715532016 7412,3553,5788 47 56 13528 15.419452887537991 0.9999999397790844 0.24201178062210515 22.028420146766024 GOTERM_BP_FAT GO:0070665~positive regulation of leukocyte proliferation 3 5.555555555555555 0.015503790715532016 7412,3553,5788 47 56 13528 15.419452887537991 0.9999999397790844 0.24201178062210515 22.028420146766024 GOTERM_BP_FAT GO:0055080~cation homeostasis 5 9.25925925925926 0.0159127623351669 5021,3553,4886,1906,5788 47 286 13528 5.031989287308437 0.999999961296523 0.24405934151141961 22.542627891321864 GOTERM_BP_FAT GO:0001775~cell activation 5 9.25925925925926 0.01609819734561397 7412,1281,5156,1906,5788 47 287 13528 5.014456223589592 0.9999999683284977 0.24309209147866861 22.774730084765093 GOTERM_BP_FAT GO:0002694~regulation of leukocyte activation 4 7.4074074074074066 0.018731460191989586 7412,7070,3553,5788 47 166 13528 6.9356575237118685 0.9999999981706396 0.269746871350535 26.001131581332615 GOTERM_BP_FAT GO:0042129~regulation of T cell proliferation 3 5.555555555555555 0.018794136084736 7412,3553,5788 47 62 13528 13.927247769389156 0.9999999982908364 0.2669733771539826 26.076363073292786 GOTERM_BP_FAT GO:0019725~cellular homeostasis 6 11.11111111111111 0.020384113743819134 5021,3553,4886,1906,5788,6005 47 466 13528 3.705962925760204 0.999999999695637 0.2825213751673976 27.961050550550137 GOTERM_BP_FAT GO:0044057~regulation of system process 5 9.25925925925926 0.02052817172198247 5021,3553,6507,4886,1906 47 309 13528 4.657439922880948 0.9999999997397241 0.2806391864426714 28.12956660317083 GOTERM_BP_FAT GO:0046883~regulation of hormone secretion 3 5.555555555555555 0.021136749134292396 3553,4886,1906 47 66 13528 13.083172147001934 0.9999999998656512 0.2841418602066095 28.83739922883691 GOTERM_BP_FAT GO:0050865~regulation of cell activation 4 7.4074074074074066 0.021508535102179833 7412,7070,3553,5788 47 175 13528 6.578966565349544 0.9999999999103206 0.28486695376712123 29.266600720109125 GOTERM_BP_FAT GO:0007242~intracellular signaling cascade 10 18.51851851851852 0.023683624485798895 695,1410,828,5999,673,5021,3553,4886,1906,5788 47 1256 13528 2.2916384333920585 0.9999999999915983 0.3053329172520969 31.729372003734856 GOTERM_BP_FAT GO:0003013~circulatory system process 4 7.4074074074074066 0.025194189693060386 5021,3043,4886,1906 47 186 13528 6.189887897506291 0.9999999999983824 0.31777485892316704 33.392189377481685 GOTERM_BP_FAT GO:0008015~blood circulation 4 7.4074074074074066 0.025194189693060386 5021,3043,4886,1906 47 186 13528 6.189887897506291 0.9999999999983824 0.31777485892316704 33.392189377481685 GOTERM_BP_FAT GO:0044092~negative regulation of molecular function 5 9.25925925925926 0.026400273582561043 7070,5999,3553,4886,5788 47 334 13528 4.308829150210218 0.9999999999995667 0.32657571862229495 34.69250348286309 GOTERM_BP_FAT GO:0048878~chemical homeostasis 6 11.11111111111111 0.029146766859380096 5021,3553,4886,1906,5788,6005 47 512 13528 3.3730053191489358 0.9999999999999786 0.3502309109666104 37.56534268789936 GOTERM_BP_FAT GO:0045859~regulation of protein kinase activity 5 9.25925925925926 0.029274644676624075 7070,5999,3553,1906,5788 47 345 13528 4.171446191797718 0.9999999999999813 0.34767086447265705 37.69617333034745 GOTERM_BP_FAT GO:0051046~regulation of secretion 4 7.4074074074074066 0.031127337352047094 5021,3553,4886,1906 47 202 13528 5.699599747208763 0.9999999999999976 0.35770605094996255 39.563051950229486 GOTERM_BP_FAT GO:0050670~regulation of lymphocyte proliferation 3 5.555555555555555 0.032337139153753365 7412,3553,5788 47 83 13528 10.403486285567801 0.9999999999999993 0.36135176803925684 40.75368203072832 GOTERM_BP_FAT GO:0043549~regulation of kinase activity 5 9.25925925925926 0.03261647217676452 7070,5999,3553,1906,5788 47 357 13528 4.031229513081828 0.9999999999999996 0.3602091456911336 41.025446843073965 GOTERM_BP_FAT GO:0032944~regulation of mononuclear cell proliferation 3 5.555555555555555 0.03305531203356653 7412,3553,5788 47 84 13528 10.279635258358661 0.9999999999999997 0.36049829872870176 41.45003845749663 GOTERM_BP_FAT GO:0070663~regulation of leukocyte proliferation 3 5.555555555555555 0.03305531203356653 7412,3553,5788 47 84 13528 10.279635258358661 0.9999999999999997 0.36049829872870176 41.45003845749663 GOTERM_BP_FAT GO:0048514~blood vessel morphogenesis 4 7.4074074074074066 0.034761298236376414 7070,7026,3553,1906 47 211 13528 5.456488857517394 1.0 0.36462765825838994 43.07357025606334 GOTERM_BP_FAT GO:0042493~response to drug 4 7.4074074074074066 0.036871751553382556 5021,3553,6507,4015 47 216 13528 5.330181245074862 1.0 0.3786558732594951 45.023648717288765 GOTERM_BP_FAT GO:0051338~regulation of transferase activity 5 9.25925925925926 0.03709923269083745 7070,5999,3553,1906,5788 47 372 13528 3.868679935941432 1.0 0.3770123718490681 45.23006351338974 GOTERM_BP_FAT GO:0009968~negative regulation of signal transduction 4 7.4074074074074066 0.03904720936773417 7070,5999,3553,5788 47 221 13528 5.20958890921344 1.0 0.389074599551271 46.96814117862471 GOTERM_BP_FAT GO:0051050~positive regulation of transport 4 7.4074074074074066 0.039935509894814834 7070,5021,3553,1906 47 223 13528 5.162866138727221 1.0 0.39251437041170123 47.74344219714266 GOTERM_BP_FAT GO:0042592~homeostatic process 7 12.962962962962962 0.04054382950334111 2877,5021,3553,4886,1906,5788,6005 47 751 13528 2.682834235204125 1.0 0.393727869349376 48.26823618544602 GOTERM_BP_FAT GO:0002253~activation of immune response 3 5.555555555555555 0.04057411197247147 7070,629,5788 47 94 13528 9.186057039384336 1.0 0.39053935306730736 48.29423120058425 GOTERM_BP_FAT GO:0043406~positive regulation of MAP kinase activity 3 5.555555555555555 0.047008787380659015 3553,1906,5788 47 102 13528 8.465581977471839 1.0 0.430490544557542 53.548608364016246 GOTERM_BP_FAT GO:0042127~regulation of cell proliferation 7 12.962962962962962 0.049066806466347965 7412,5320,5156,945,3553,1906,5788 47 787 13528 2.5601124658682313 1.0 0.4376362899497581 55.12057472194013 GOTERM_BP_FAT GO:0008284~positive regulation of cell proliferation 5 9.25925925925926 0.05147308759984519 7412,5156,3553,1906,5788 47 414 13528 3.476205159831432 1.0 0.4501785790747824 56.8952985069815 GOTERM_BP_FAT GO:0010648~negative regulation of cell communication 4 7.4074074074074066 0.051899800394626244 7070,5999,3553,5788 47 248 13528 4.642415923129719 1.0 0.449487143624119 57.203065629868775 GOTERM_BP_FAT GO:0043408~regulation of MAPKKK cascade 3 5.555555555555555 0.05292504428385492 3553,1906,5788 47 109 13528 7.921920749560803 1.0 0.45265657521815994 57.93412637067683 GOTERM_BP_FAT GO:0010564~regulation of cell cycle process 3 5.555555555555555 0.0573054956530903 3553,1906,5788 47 114 13528 7.574468085106383 1.0 0.4629594167836182 60.927818589411096 GOTERM_BP_FAT GO:0051173~positive regulation of nitrogen compound metabolic process 6 11.11111111111111 0.06631433228738538 5156,7026,3553,3043,1906,5788 47 644 13528 2.68164398044139 1.0 0.5077675296841472 66.46815034657382 GOTERM_BP_FAT GO:0042325~regulation of phosphorylation 5 9.25925925925926 0.07297204387090589 7070,5999,3553,1906,5788 47 466 13528 3.088302438133504 1.0 0.5393889521440735 70.07969474109778 GOTERM_BP_FAT GO:0050804~regulation of synaptic transmission 3 5.555555555555555 0.07796882538600976 5021,6507,1906 47 136 13528 6.349186483103879 1.0 0.5607058038704307 72.54715556320109 GOTERM_BP_FAT GO:0019220~regulation of phosphate metabolic process 5 9.25925925925926 0.08182477759907406 7070,5999,3553,1906,5788 47 485 13528 2.9673173941653874 1.0 0.57552056619956 74.31947059303647 GOTERM_BP_FAT GO:0051174~regulation of phosphorus metabolic process 5 9.25925925925926 0.08182477759907406 7070,5999,3553,1906,5788 47 485 13528 2.9673173941653874 1.0 0.57552056619956 74.31947059303647 GOTERM_BP_FAT GO:0007155~cell adhesion 6 11.11111111111111 0.08768252633814311 7412,1281,7070,945,10418,5788 47 700 13528 2.467112462006079 1.0 0.5917122118064031 76.80788806171898 GOTERM_BP_FAT GO:0022610~biological adhesion 6 11.11111111111111 0.08809410048996613 7412,1281,7070,945,10418,5788 47 701 13528 2.463593043372689 1.0 0.590166440541759 76.97394227050434 GOTERM_BP_FAT GO:0051969~regulation of transmission of nerve impulse 3 5.555555555555555 0.08905887621540617 5021,6507,1906 47 147 13528 5.874077290490663 1.0 0.5910305340106511 77.35883475819348 GOTERM_BP_FAT GO:0001525~angiogenesis 3 5.555555555555555 0.09008958641954389 7070,3553,1906 47 148 13528 5.834387579068429 1.0 0.592162612411822 77.76336483327114 GOTERM_BP_FAT GO:0031644~regulation of neurological system process 3 5.555555555555555 0.09529642522101195 5021,6507,1906 47 153 13528 5.64372131831456 1.0 0.6105367713071908 79.70543759671737 GOTERM_BP_FAT GO:0010647~positive regulation of cell communication 4 7.4074074074074066 0.10082378998136374 5021,3553,6507,5788 47 329 13528 3.4994503007178426 1.0 0.6259203037906597 81.59243443498934 GOTERM_BP_FAT GO:0001932~regulation of protein amino acid phosphorylation 3 5.555555555555555 0.11693860543346996 3553,1906,5788 47 173 13528 4.9912679867175 1.0 0.6710763542464311 86.19851305638677 GOTERM_BP_FAT GO:0051130~positive regulation of cellular component organization 3 5.555555555555555 0.12592070395528462 5021,3553,1906 47 181 13528 4.770659456917833 1.0 0.6967849714625384 88.272119058937 GOTERM_BP_FAT GO:0044093~positive regulation of molecular function 5 9.25925925925926 0.13713989721578065 7070,800,3553,1906,5788 47 586 13528 2.455885556604459 1.0 0.7266626670525078 90.4528142113603 GOTERM_BP_FAT GO:0045860~positive regulation of protein kinase activity 3 5.555555555555555 0.175410553210257 3553,1906,5788 47 223 13528 3.8721496040454153 1.0 0.796237644848153 95.3642628115751 GOTERM_BP_FAT GO:0033674~positive regulation of kinase activity 3 5.555555555555555 0.1851805395380181 3553,1906,5788 47 231 13528 3.738049184857695 1.0 0.8104916232898012 96.16569233999334 GOTERM_BP_FAT GO:0051347~positive regulation of transferase activity 3 5.555555555555555 0.19626733989240022 3553,1906,5788 47 240 13528 3.5978723404255315 1.0 0.8235759002846907 96.9172446319901 GOTERM_BP_FAT GO:0045321~leukocyte activation 3 5.555555555555555 0.19874330147250457 7412,1906,5788 47 242 13528 3.568137858273255 1.0 0.8255872519997713 97.06504813259414 GOTERM_BP_FAT GO:0010627~regulation of protein kinase cascade 3 5.555555555555555 0.20744023356368888 3553,1906,5788 47 249 13528 3.4678287618559342 1.0 0.8356234211509013 97.53325723129684 GOTERM_BP_FAT GO:0007610~behavior 4 7.4074074074074066 0.21319907252721412 5021,3553,6507,4886 47 469 13528 2.454838270652815 1.0 0.8404564539566413 97.80371811096899 GOTERM_BP_FAT GO:0016337~cell-cell adhesion 3 5.555555555555555 0.2413305500730002 7412,7070,5788 47 276 13528 3.128584643848288 1.0 0.8755872236571898 98.7700602895255 GOTERM_BP_FAT GO:0043085~positive regulation of catalytic activity 4 7.4074074074074066 0.2594872265840371 7070,3553,1906,5788 47 520 13528 2.214075286415712 1.0 0.8947037409999836 99.16371133773812 GOTERM_BP_FAT GO:0031399~regulation of protein modification process 3 5.555555555555555 0.2653688662880838 3553,1906,5788 47 295 13528 2.9270825820411104 1.0 0.8991952753555598 99.26344413033054 GOTERM_BP_FAT GO:0007268~synaptic transmission 3 5.555555555555555 0.26916981874630375 5021,6507,4885 47 298 13528 2.8976153077252604 1.0 0.9014276615777878 99.32184364280538 GOTERM_BP_FAT GO:0001501~skeletal system development 3 5.555555555555555 0.29576470110995157 1281,5156,1906 47 319 13528 2.706863202827986 1.0 0.9196077136305283 99.62419332337896 GOTERM_BP_FAT GO:0051726~regulation of cell cycle 3 5.555555555555555 0.3109205115010065 3553,1906,5788 47 331 13528 2.6087291894324096 1.0 0.9274916854552742 99.73423285253959 GOTERM_BP_FAT GO:0019226~transmission of nerve impulse 3 5.555555555555555 0.33479310962244646 5021,6507,4885 47 350 13528 2.467112462006079 1.0 0.9412784930535558 99.84841567184617 GOTERM_BP_FAT GO:0050890~cognition 5 9.25925925925926 0.3737708651131038 157506,5021,3553,6507,4886 47 909 13528 1.5832221520024343 1.0 0.9597586773541595 99.94204970533409 GOTERM_BP_FAT GO:0050877~neurological system process 6 11.11111111111111 0.39444697751485813 157506,5021,3553,6507,4885,4886 47 1210 13528 1.4272551433093017 1.0 0.9673287218882618 99.96604862523047 GOTERM_BP_FAT GO:0006955~immune response 4 7.4074074074074066 0.41868757742574625 5553,3553,629,5788 47 690 13528 1.6685784767190872 1.0 0.974684449305478 99.98228611833927 GOTERM_BP_FAT GO:0032268~regulation of cellular protein metabolic process 3 5.555555555555555 0.48269233601879213 3553,1906,5788 47 474 13528 1.8217075141395096 1.0 0.9861049920775404 99.99723584905692 GOTERM_BP_FAT GO:0010604~positive regulation of macromolecule metabolic process 4 7.4074074074074066 0.5645413517906037 5156,7026,3553,5788 47 857 13528 1.3434295786886465 1.0 0.9951495698061555 99.99982202096282 GOTERM_BP_FAT GO:0007166~cell surface receptor linked signal transduction 7 12.962962962962962 0.6180295911972736 1281,7070,5156,5021,4886,1906,5788 47 1856 13528 1.085564930300807 1.0 0.9978272802019885 99.99997792074731 GOTERM_BP_FAT GO:0045935~positive regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process 3 5.555555555555555 0.6331705617425778 5156,7026,5788 47 624 13528 1.38379705400982 1.0 0.9982557590507021 99.9999884052888 GOTERM_BP_FAT GO:0007600~sensory perception 3 5.555555555555555 0.7709620164000096 157506,6507,4886 47 810 13528 1.0660362490149724 1.0 0.9998902617336282 99.99999999359123 GOTERM_BP_FAT GO:0007186~G-protein coupled receptor protein signaling pathway 3 5.555555555555555 0.9045063279387571 5021,4886,1906 47 1123 13528 0.7689130558344859 1.0 0.9999994672187325 99.99999999999999 Annotation Cluster 6 Enrichment Score: 1.3592005006484587 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0006916~anti-apoptosis 5 9.25925925925926 0.005170190372642541 1410,673,4353,3553,5055 47 206 13528 6.986159884321421 0.9959754918954242 0.14579280920445825 7.923171490059122 GOTERM_BP_FAT GO:0043066~negative regulation of apoptosis 5 9.25925925925926 0.0317607324369962 1410,673,4353,3553,5055 47 354 13528 4.065392475057099 0.9999999999999988 0.35981383260919675 40.18917836219692 GOTERM_BP_FAT GO:0043069~negative regulation of programmed cell death 5 9.25925925925926 0.03319450335991771 1410,673,4353,3553,5055 47 359 13528 4.008771409944882 0.9999999999999998 0.3581737780872838 41.5841109334085 GOTERM_BP_FAT GO:0060548~negative regulation of cell death 5 9.25925925925926 0.03348578335838204 1410,673,4353,3553,5055 47 360 13528 3.9976359338061465 0.9999999999999998 0.3572116759773474 41.86374838732833 GOTERM_BP_FAT GO:0042981~regulation of apoptosis 7 12.962962962962962 0.053455290134839566 695,1410,673,4353,3553,5788,5055 47 804 13528 2.5059807346247482 1.0 0.4526181894299085 58.30761572108869 GOTERM_BP_FAT GO:0043067~regulation of programmed cell death 7 12.962962962962962 0.05560223036539585 695,1410,673,4353,3553,5788,5055 47 812 13528 2.4812912692589877 1.0 0.45927246914856223 59.78832185477416 GOTERM_BP_FAT GO:0010941~regulation of cell death 7 12.962962962962962 0.05642088656677141 695,1410,673,4353,3553,5788,5055 47 815 13528 2.4721576817647826 1.0 0.4609347777710142 60.339841024785066 GOTERM_BP_FAT GO:0043933~macromolecular complex subunit organization 4 7.4074074074074066 0.4370684949859479 1410,5320,673,4353 47 710 13528 1.6215762661072821 1.0 0.9775696158413113 99.98938057775146 Annotation Cluster 7 Enrichment Score: 1.2786161186233802 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0007267~cell-cell signaling 7 12.962962962962962 0.015381673037560162 945,5021,3553,6507,4258,4885,1906 47 600 13528 3.3580141843971627 0.9999999312833909 0.24387383536774043 21.8742601134708 GOTERM_BP_FAT GO:0046394~carboxylic acid biosynthetic process 3 5.555555555555555 0.09740338150425328 6507,4258,1906 47 155 13528 5.570899107755662 1.0 0.6157552993238813 80.44515583175867 GOTERM_BP_FAT GO:0016053~organic acid biosynthetic process 3 5.555555555555555 0.09740338150425328 6507,4258,1906 47 155 13528 5.570899107755662 1.0 0.6157552993238813 80.44515583175867 Annotation Cluster 8 Enrichment Score: 1.0592068817572682 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0045429~positive regulation of nitric oxide biosynthetic process 3 5.555555555555555 0.002279687258581288 3553,3043,1906 47 21 13528 41.118541033434646 0.9118183372046201 0.12620429704814295 3.569204876290022 GOTERM_BP_FAT GO:0045428~regulation of nitric oxide biosynthetic process 3 5.555555555555555 0.003761252522712781 3553,3043,1906 47 27 13528 31.98108747044917 0.9818578591757724 0.13800056189258525 5.824434062273998 GOTERM_BP_FAT GO:0008284~positive regulation of cell proliferation 5 9.25925925925926 0.05147308759984519 7412,5156,3553,1906,5788 47 414 13528 3.476205159831432 1.0 0.4501785790747824 56.8952985069815 GOTERM_BP_FAT GO:0051173~positive regulation of nitrogen compound metabolic process 6 11.11111111111111 0.06631433228738538 5156,7026,3553,3043,1906,5788 47 644 13528 2.68164398044139 1.0 0.5077675296841472 66.46815034657382 GOTERM_BP_FAT GO:0031328~positive regulation of cellular biosynthetic process 5 9.25925925925926 0.20269297862294378 5156,7026,3553,3043,1906 47 685 13528 2.1009473520733035 1.0 0.8300328862725678 97.2871522981994 GOTERM_BP_FAT GO:0009891~positive regulation of biosynthetic process 5 9.25925925925926 0.20980479252213843 5156,7026,3553,3043,1906 47 695 13528 2.0707178937700905 1.0 0.8372513719111562 97.64787934333825 GOTERM_BP_FAT GO:0010604~positive regulation of macromolecule metabolic process 4 7.4074074074074066 0.5645413517906037 5156,7026,3553,5788 47 857 13528 1.3434295786886465 1.0 0.9951495698061555 99.99982202096282 GOTERM_BP_FAT GO:0045935~positive regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process 3 5.555555555555555 0.6331705617425778 5156,7026,5788 47 624 13528 1.38379705400982 1.0 0.9982557590507021 99.9999884052888 GOTERM_BP_FAT GO:0010557~positive regulation of macromolecule biosynthetic process 3 5.555555555555555 0.6589797908233601 5156,7026,3553 47 654 13528 1.3203201249268006 1.0 0.9988559369379044 99.99999637169805 Annotation Cluster 9 Enrichment Score: 0.9547330463793282 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0007243~protein kinase cascade 8 14.814814814814813 2.3077972847647784E-4 695,1410,5999,673,5021,3553,1906,5788 47 370 13528 6.223346751006325 0.21774771032311646 0.04010335480012295 0.3668755973940119 GOTERM_BP_FAT GO:0042981~regulation of apoptosis 7 12.962962962962962 0.053455290134839566 695,1410,673,4353,3553,5788,5055 47 804 13528 2.5059807346247482 1.0 0.4526181894299085 58.30761572108869 GOTERM_BP_FAT GO:0043067~regulation of programmed cell death 7 12.962962962962962 0.05560223036539585 695,1410,673,4353,3553,5788,5055 47 812 13528 2.4812912692589877 1.0 0.45927246914856223 59.78832185477416 GOTERM_BP_FAT GO:0010941~regulation of cell death 7 12.962962962962962 0.05642088656677141 695,1410,673,4353,3553,5788,5055 47 815 13528 2.4721576817647826 1.0 0.4609347777710142 60.339841024785066 GOTERM_BP_FAT GO:0006468~protein amino acid phosphorylation 5 9.25925925925926 0.19009277110129058 695,5156,673,3553,5788 47 667 13528 2.157644581964337 1.0 0.8172182524305142 96.5176930567707 GOTERM_BP_FAT GO:0016310~phosphorylation 5 9.25925925925926 0.2882649969226607 695,5156,673,3553,5788 47 800 13528 1.798936170212766 1.0 0.9146770287191174 99.55513704592889 GOTERM_BP_FAT GO:0006796~phosphate metabolic process 5 9.25925925925926 0.4241356409666739 695,5156,673,3553,5788 47 973 13528 1.4790842098357786 1.0 0.9756805497047776 99.98475274851013 GOTERM_BP_FAT GO:0006793~phosphorus metabolic process 5 9.25925925925926 0.4241356409666739 695,5156,673,3553,5788 47 973 13528 1.4790842098357786 1.0 0.9756805497047776 99.98475274851013 GOTERM_BP_FAT GO:0043065~positive regulation of apoptosis 3 5.555555555555555 0.4322058394584751 695,3553,5788 47 430 13528 2.0081147946561106 1.0 0.9773489188841976 99.98782174217826 GOTERM_BP_FAT GO:0043068~positive regulation of programmed cell death 3 5.555555555555555 0.4357277805855829 695,3553,5788 47 433 13528 1.9942017591273156 1.0 0.9777464320927582 99.98897057589737 GOTERM_BP_FAT GO:0010942~positive regulation of cell death 3 5.555555555555555 0.4380695755436831 695,3553,5788 47 435 13528 1.98503301540719 1.0 0.9773045034531666 99.98967735414037 Annotation Cluster 10 Enrichment Score: 0.8973591997304606 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0007584~response to nutrient 3 5.555555555555555 0.08194766537142452 3553,2029,629 47 140 13528 6.167781155015198 1.0 0.5726766805937503 74.37414981263693 GOTERM_BP_FAT GO:0031667~response to nutrient levels 3 5.555555555555555 0.14435731040907573 3553,2029,629 47 197 13528 4.383194729452424 1.0 0.7375627435910969 91.64813879391835 GOTERM_BP_FAT GO:0009991~response to extracellular stimulus 3 5.555555555555555 0.17176988842154983 3553,2029,629 47 220 13528 3.92495164410058 1.0 0.791248156409674 95.02736625283714 Annotation Cluster 11 Enrichment Score: 0.8482026879872226 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0007423~sensory organ development 4 7.4074074074074066 0.04266218472677767 1410,7070,5156,1906 47 229 13528 5.027594536839171 1.0 0.4027601838380097 50.05743635498081 GOTERM_BP_FAT GO:0030334~regulation of cell migration 3 5.555555555555555 0.11251322839878178 7070,5156,1906 47 169 13528 5.10940450711318 1.0 0.6591381602658934 85.05492662525154 GOTERM_BP_FAT GO:0040012~regulation of locomotion 3 5.555555555555555 0.13853319426209318 7070,5156,1906 47 192 13528 4.497340425531914 1.0 0.727607463761718 90.69537590657642 GOTERM_BP_FAT GO:0051270~regulation of cell motion 3 5.555555555555555 0.1396936818912761 7070,5156,1906 47 193 13528 4.47403814353434 1.0 0.7279035608553106 90.89298609733099 GOTERM_BP_FAT GO:0007166~cell surface receptor linked signal transduction 7 12.962962962962962 0.6180295911972736 1281,7070,5156,5021,4886,1906,5788 47 1856 13528 1.085564930300807 1.0 0.9978272802019885 99.99997792074731 Annotation Cluster 12 Enrichment Score: 0.5954378608875407 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0006928~cell motion 4 7.4074074074074066 0.21854655019594957 7412,7026,800,3553 47 475 13528 2.4238297872340424 1.0 0.8465158063533929 98.0297408818388 GOTERM_BP_FAT GO:0016477~cell migration 3 5.555555555555555 0.2413305500730002 7412,7026,3553 47 276 13528 3.128584643848288 1.0 0.8755872236571898 98.7700602895255 GOTERM_BP_FAT GO:0051674~localization of cell 3 5.555555555555555 0.2805731765439311 7412,7026,3553 47 307 13528 2.8126689306258226 1.0 0.9092669416265806 99.47208118499654 GOTERM_BP_FAT GO:0048870~cell motility 3 5.555555555555555 0.2805731765439311 7412,7026,3553 47 307 13528 2.8126689306258226 1.0 0.9092669416265806 99.47208118499654 Annotation Cluster 13 Enrichment Score: 0.0171267008580914 Category Term Count % PValue Genes List Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR GOTERM_BP_FAT GO:0006355~regulation of transcription, DNA-dependent 4 7.4074074074074066 0.9511112533691608 5151,2625,7026,7738 47 1773 13528 0.6493621821410999 1.0 0.9999999903486564 100.0 GOTERM_BP_FAT GO:0051252~regulation of RNA metabolic process 4 7.4074074074074066 0.95632712085812 5151,2625,7026,7738 47 1813 13528 0.6350353827557476 1.0 0.9999999945988295 100.0 GOTERM_BP_FAT GO:0045449~regulation of transcription 6 11.11111111111111 0.9569067645070283 5151,2625,7026,3553,55885,7738 47 2601 13528 0.66396721391936 1.0 0.9999999944488585 100.0 GOTERM_BP_FAT GO:0006350~transcription 4 7.4074074074074066 0.9812638043304253 2625,7026,55885,7738 47 2101 13528 0.5479862679372538 1.0 0.999999999958641 100.0