Hi all i am working on some data obtained from smd database which is basically a time series data having human fibroblast infected by a pathogen t. gondii . expression was recorded as follows "a time course was performed during which RNA was purified from HFFs that were mock- or parasite-infected for 1, 2, 4, 6, and 24 hpi with T. gondii tachyzoites. To minimize host cell lysis following parasite egress, the time course was limited to 24 h" To perform differential expression on this data using limma i have to build linear model or matrix. how can i make matrix for this kind of data? As i am new to bioconductor as well as microarray data analysis please tell me if there is another way to do the analysis of this kind of data??????? it would be useful if u kindly suggest articles, research papers or tutorials on this . with regards -- BUDHAYASH GAUTAM Assistant Professor Department Of Computational Biology And Bioinformatics, Jacob School of Biotechnology & Bioengineering (JSBB) Sam Higginbottom Institute of Agriculture, Technology and Sciences (SHIATS)- Deemed University, Naini, Allahabad. U.P. 211007

You may try Short Time-series Expression Miner (STEM)<http: www.cs.cmu.edu="" ~jernst="" stem=""/>