Complete newbie - reconstruct array image with only data file?
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D M ▴ 40
@d-m-4384
Last seen 9.6 years ago
Hi all So sorry to be a total newbie, but i'm just finding my feet with R. I heard on the grapevine i could recreate a scanned microarray image, or at least get a good graphic of it from a just a data file. I have .txt files for illumina beadarrays but no images cos the service we used didn't send them. The 'beadarray' package seems to require TIFFs to get the graphic output. Does anone know of a way/a package i can use to rebuild the image? Many thanks! [[alternative HTML version deleted]]
Microarray Microarray • 890 views
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Mark Dunning ★ 1.1k
@mark-dunning-3319
Last seen 13 months ago
Sheffield, Uk
Hi, I'm not clear what you mean by rebuilding the image? beadarray is able to create false-colour representations of the array surface in order to check for spatial artefacts, if that's what you mean. Please see the beadarray vignette for details Regards, Mark On Thu, Dec 9, 2010 at 3:07 PM, D M <srafmonkey at="" yahoo.co.uk=""> wrote: > Hi all > > So sorry to be a total newbie, but i'm just finding my feet with R. > > ?I heard on the grapevine i could recreate a scanned microarray image, or at > least get a good graphic of it from a just a data file. > > I have .txt files for illumina beadarrays but no images cos the service we > used didn't send them. The 'beadarray' package seems to require TIFFs to get > the graphic output. > > Does anone know of a way/a package i can use to rebuild the image? > > Many thanks! > > > > > > > ? ? ? ?[[alternative HTML version deleted]] > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >
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On third viewing, i do need .tifs to get my graphics working. What i need to know is - Can i make the graphics (with this package or by any other means) using only .txt file, since these are the only files i have? Cheers! --- On Thu, 9/12/10, Mark Dunning <mark.dunning@gmail.com> wrote: From: Mark Dunning <mark.dunning@gmail.com> Subject: Re: [BioC] Complete newbie - reconstruct array image with only data file? To: "D M" <srafmonkey@yahoo.co.uk> Cc: bioconductor@r-project.org Date: Thursday, 9 December, 2010, 17:14 Hi, I'm not clear what you mean by rebuilding the image? beadarray is able to create false-colour representations of the array surface in order to check for spatial artefacts, if that's what you mean. Please see the beadarray vignette for details Regards, Mark On Thu, Dec 9, 2010 at 3:07 PM, D M <srafmonkey@yahoo.co.uk> wrote: > Hi all > > So sorry to be a total newbie, but i'm just finding my feet with R. > >  I heard on the grapevine i could recreate a scanned microarray image, or at > least get a good graphic of it from a just a data file. > > I have .txt files for illumina beadarrays but no images cos the service we > used didn't send them. The 'beadarray' package seems to require TIFFs to get > the graphic output. > > Does anone know of a way/a package i can use to rebuild the image? > > Many thanks! > > > > > > >        [[alternative HTML version deleted]] > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > [[alternative HTML version deleted]]
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Hi, On Thu, Dec 9, 2010 at 4:43 PM, D M <srafmonkey at="" yahoo.co.uk=""> wrote: > On third viewing, i do need .tifs to get my graphics working. > > What i need to know is - > > Can i make the graphics (with this package or by any other means) using only .txt file, since these are the only files i have? If you have a matrix of numbers (presumably your probe intensities), you can make an image using the `image` function. See ?image It sounds like your job is to parse your txt file for the probe intensities, and store those values in the appropriate place of your "chip matrix," so to speak. -steve -- Steve Lianoglou Graduate Student: Computational Systems Biology ?| Memorial Sloan-Kettering Cancer Center ?| Weill Medical College of Cornell University Contact Info: http://cbio.mskcc.org/~lianos/contact
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