question about data analysis of Genechip miRNA
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@james-anderson-1641
Last seen 9.6 years ago
Hi, I need to analyze some Genechip miRNA data in my work, can anybody recommend some packages or methods? or everything is the same as the Affy mRNA Genechip data? Thanks, -James [[alternative HTML version deleted]]
miRNA miRNA • 1.1k views
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Guido Hooiveld ★ 3.9k
@guido-hooiveld-2020
Last seen 5 hours ago
Wageningen University, Wageningen, the …
Hi James, I analyzed my Affymetrix miRNA arrays using a custom, remapped CDF, with the packages 'affy' and 'limma', just like the regular Affymetrix mRNA arrays. Note that this workflow will also do using the default Affy CDF (which is available on BioC). Alternatively, you may want to use the package 'xps'. I used quantile normalization because my samples were all from the same tissue that underwent a relatively mild intervention, so I expected most of the miRNAs to remain constantly expressed (in contrast to e.g. comparing different tissues or tumors). You may want to check these threads for a bit more info on this and related strategies (links point to the starting post of a topic; be sure to click through the complete threads): http://article.gmane.org/gmane.science.biology.informatics.conductor/3 1837 http://article.gmane.org/gmane.science.biology.informatics.conductor/2 9921 http://article.gmane.org/gmane.science.biology.informatics.conductor/3 2287 http://article.gmane.org/gmane.science.biology.informatics.conductor/2 4682 Hope this will get you started, Guido ------------------------------------------------ Guido Hooiveld, PhD Nutrition, Metabolism & Genomics Group Division of Human Nutrition Wageningen University Biotechnion, Bomenweg 2 NL-6703 HD Wageningen the Netherlands tel: (+)31 317 485788 fax: (+)31 317 483342 email: guido.hooiveld at wur.nl internet: http://nutrigene.4t.com http://www.researcherid.com/rid/F-4912-2010 > -----Original Message----- > From: bioconductor-bounces at r-project.org > [mailto:bioconductor-bounces at r-project.org] On Behalf Of > James Anderson > Sent: Tuesday, January 11, 2011 20:14 > To: bioconductor > Subject: [BioC] question about data analysis of Genechip miRNA > > Hi, > > I need to analyze some Genechip miRNA data in my work, can > anybody recommend some packages or methods? or everything is > the same as the Affy mRNA Genechip data? > > Thanks, > > -James > > > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > >
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library(hugene10sttranscriptcluster.db) > ls("package:hugene10sttranscriptcluster.db") [1] "hugene10sttranscriptcluster" [2] "hugene10sttranscriptclusterACCNUM" [3] "hugene10sttranscriptclusterALIAS2PROBE" [4] "hugene10sttranscriptclusterCHR" [5] "hugene10sttranscriptclusterCHRLENGTHS" [6] "hugene10sttranscriptclusterCHRLOC" [7] "hugene10sttranscriptclusterCHRLOCEND" [8] "hugene10sttranscriptcluster_dbconn" [9] "hugene10sttranscriptcluster_dbfile" [10] "hugene10sttranscriptcluster_dbInfo" [11] "hugene10sttranscriptcluster_dbschema" [12] "hugene10sttranscriptclusterENSEMBL" [13] "hugene10sttranscriptclusterENSEMBL2PROBE" [14] "hugene10sttranscriptclusterENTREZID" [15] "hugene10sttranscriptclusterENZYME" [16] "hugene10sttranscriptclusterENZYME2PROBE" [17] "hugene10sttranscriptclusterGENENAME" [18] "hugene10sttranscriptclusterGO" [19] "hugene10sttranscriptclusterGO2ALLPROBES" [20] "hugene10sttranscriptclusterGO2PROBE" [21] "hugene10sttranscriptclusterMAP" [22] "hugene10sttranscriptclusterMAPCOUNTS" [23] "hugene10sttranscriptclusterOMIM" [24] "hugene10sttranscriptclusterORGANISM" [25] "hugene10sttranscriptclusterORGPKG" [26] "hugene10sttranscriptclusterPATH" [27] "hugene10sttranscriptclusterPATH2PROBE" [28] "hugene10sttranscriptclusterPFAM" [29] "hugene10sttranscriptclusterPMID" [30] "hugene10sttranscriptclusterPMID2PROBE" [31] "hugene10sttranscriptclusterPROSITE" [32] "hugene10sttranscriptclusterREFSEQ" [33] "hugene10sttranscriptclusterSYMBOL" [34] "hugene10sttranscriptclusterUNIGENE" [35] "hugene10sttranscriptclusterUNIPROT" Above, I note that GeneBank ids are not directly supported for my chip. Darn. I am therefore using an online converter to map a list of GenBank accessions to ENTREZ gene ids because it looks tricky to use the entrezGeneByID function in annotate for a list of IDS. Ami I missing something? Best, Tom
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Hi Thomas, You can get GeneBank IDs, but they are jumbled together with other sorts of accessions inside of the ACCNUM mappings. If you just want all possible accessions, you can do it like this: prbs = c("8180408","8180388") egs = unlist2(mget(prbs, hugene10sttranscriptclusterENTREZID, ifnotfound=NA)) mget(egs, org.Hs.egACCNUM, ifnotfound=NA) ## OR a more tabular way: prbs = c("8180408","8180388") merge(toTable(hugene10sttranscriptclusterENTREZID[prbs]), toTable(org.Hs.egACCNUM)) Regardless of which method you use, the result is the same and will net you all the accessions for these genes. At this point, it is pretty easy to filter out the refseq IDs (for example) by grepping for "_". But please notice however that you want to use the ACCNUM mapping from org.Hs.eg to get this. This mapping is NOT the same as the ACCNUM mapping in your chip package. That is because the one in your chip package only holds accessions that were used to actually make the association between the transcript IDs and the Entrez Gene IDs in that package. This makes it conceptually a bit different from all the other mappings. In contrast, the one in org.Hs.eg holds all of the accessions that are affiliated with a given gene. Hope that this helps you, Marc On 01/13/2011 01:25 PM, Thomas Hampton wrote: > > library(hugene10sttranscriptcluster.db) > > > ls("package:hugene10sttranscriptcluster.db") > [1] "hugene10sttranscriptcluster" > [2] "hugene10sttranscriptclusterACCNUM" > [3] "hugene10sttranscriptclusterALIAS2PROBE" > [4] "hugene10sttranscriptclusterCHR" > [5] "hugene10sttranscriptclusterCHRLENGTHS" > [6] "hugene10sttranscriptclusterCHRLOC" > [7] "hugene10sttranscriptclusterCHRLOCEND" > [8] "hugene10sttranscriptcluster_dbconn" > [9] "hugene10sttranscriptcluster_dbfile" > [10] "hugene10sttranscriptcluster_dbInfo" > [11] "hugene10sttranscriptcluster_dbschema" > [12] "hugene10sttranscriptclusterENSEMBL" > [13] "hugene10sttranscriptclusterENSEMBL2PROBE" > [14] "hugene10sttranscriptclusterENTREZID" > [15] "hugene10sttranscriptclusterENZYME" > [16] "hugene10sttranscriptclusterENZYME2PROBE" > [17] "hugene10sttranscriptclusterGENENAME" > [18] "hugene10sttranscriptclusterGO" > [19] "hugene10sttranscriptclusterGO2ALLPROBES" > [20] "hugene10sttranscriptclusterGO2PROBE" > [21] "hugene10sttranscriptclusterMAP" > [22] "hugene10sttranscriptclusterMAPCOUNTS" > [23] "hugene10sttranscriptclusterOMIM" > [24] "hugene10sttranscriptclusterORGANISM" > [25] "hugene10sttranscriptclusterORGPKG" > [26] "hugene10sttranscriptclusterPATH" > [27] "hugene10sttranscriptclusterPATH2PROBE" > [28] "hugene10sttranscriptclusterPFAM" > [29] "hugene10sttranscriptclusterPMID" > [30] "hugene10sttranscriptclusterPMID2PROBE" > [31] "hugene10sttranscriptclusterPROSITE" > [32] "hugene10sttranscriptclusterREFSEQ" > [33] "hugene10sttranscriptclusterSYMBOL" > [34] "hugene10sttranscriptclusterUNIGENE" > [35] "hugene10sttranscriptclusterUNIPROT" > > Above, I note that GeneBank ids are not directly supported for my > chip. Darn. > > I am therefore using an online converter to map a list of GenBank > accessions to ENTREZ gene ids because it looks tricky to use > the entrezGeneByID function in annotate for a list of IDS. > > Ami I missing something? > > Best, > > Tom > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor
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