Entering edit mode
Peevi Ijkl
▴
170
@peevi-ijkl-4360
Last seen 10.2 years ago
Hi list..
I am trying to create an expression set object..here is what i did so
far..
pd <- read.AnnotatedDataFrame( "target.txt", header=T, row.names=1,
sep=";" )
pData(pd)
expression_data <- ReadAffy( filenames = rownames (pData(pd)) )
expression_data
rma_expression_data <-rma(expression_data)
rma_data <- exprs(rma_expression_data)
now i want to create an expression set object from the above using the
following:
ALLSet <- new("ExpressionSet", exprs =rma_data, phenoData = pd,
+ annotation = "hgu95av2")
but I am getting the following error when i use the above
Error in validObject(.Object) :
invalid class "ExpressionSet" object: 1: row numbers differ for
assayData
members
invalid class "ExpressionSet" object: 2: sample numbers differ for
assayData
members
while if i use rma_expression_data at the highlighted area am getting
the
following error:
Error in function (classes, fdef, mtable) :
unable to find an inherited method for function
"annotatedDataFrameFrom", for
signature "ExpressionSet"
any help would be appreciated.
thanks
peevi
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