Hi Daniel, The error message about 'locale' suggests that this is to do with conflicts between different language versions of R. My guess is that you might be using a version of R compiled for a non-English language (such as French) but trying to read a GenePix file encoded in a way that is not compatible with that language. If I'm right, it's an R issue rather than a limma issue. You could clarify this by giving us system information: sessionInfo() There are a number of previous posts on these issues, see for example: https://stat.ethz.ch/pipermail/bioconductor/2007-December/020549.html https://stat.ethz.ch/pipermail/bioconductor/2007-July/018204.html https://stat.ethz.ch/pipermail/bioc-devel/2005-April/000070.html Best wishes Gordon > Date: Tue, 22 Feb 2011 15:50:38 -0800 > From: Daniel Fortin <danielfortin86 at="" hotmail.com=""> > To: <bioconductor at="" stat.math.ethz.ch=""> > Subject: [BioC] LIMMA > > I have a problem when running LIMMA. I get the following error message: > Error in grep(protectMetachar(allcnames[i]), text.to.search) : > regular expression is invalid in this locale > Calls: read.maimages -> read.columns -> grep > > The code is below: > >> library(limma) >> >> targets <-readTargets(file="Targets.txt") >> RG <-read.maimages(targets$Filename, source="genepix",wt.fun=wtflags(weight=0,cutoff=-50)) > > Does anyone know how to fix this error? > > Thanks, > > D ______________________________________________________________________ The information in this email is confidential and intend...{{dropped:4}}