edgeR: calcNormFactors
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Naomi Altman ★ 6.0k
@naomi-altman-380
Last seen 2.9 years ago
United States
It looks like calcNormFactors wants a matrix, not a DGEList. Did I do something wrong? d=DGEList(counts=TotalReads, group=treatments,genes=rownames(TotalReads)) Calculating library sizes from column totals. dNorm=calcNormFactors(d) Error in calcNormFactors(d) : 'dataMatrix' needs to be a matrix Naomi
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João Moura ▴ 160
@joao-moura-4505
Last seen 9.6 years ago
Hi Naomi, just use as.matrix(TotalReads) instead ;) Cheers On Fri, Mar 11, 2011 at 8:38 PM, Naomi Altman <naomi@stat.psu.edu> wrote: > It looks like calcNormFactors wants a matrix, not a DGEList. Did I do > something wrong? > > d=DGEList(counts=TotalReads, group=treatments,genes=rownames(TotalReads)) > > Calculating library sizes from column totals. > > dNorm=calcNormFactors(d) > > Error in calcNormFactors(d) : 'dataMatrix' needs to be a matrix > > Naomi > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > -- João Moura [[alternative HTML version deleted]]
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Hi Naomi, I think this error is associated with old version of edgeR. Try upgrading to the latest version of edgeR. That's how I was able to get rid of it. Neel On Mar 11, 2011, at 3:24 PM, Jo?o Moura wrote: > Hi Naomi, > > just use as.matrix(TotalReads) instead ;) > > Cheers > > On Fri, Mar 11, 2011 at 8:38 PM, Naomi Altman <naomi at="" stat.psu.edu=""> wrote: > >> It looks like calcNormFactors wants a matrix, not a DGEList. Did I do >> something wrong? >> >> d=DGEList(counts=TotalReads, group=treatments,genes=rownames(TotalReads)) >> >> Calculating library sizes from column totals. >> >> dNorm=calcNormFactors(d) >> >> Error in calcNormFactors(d) : 'dataMatrix' needs to be a matrix >> >> Naomi >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > > > > -- > Jo?o Moura > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor Neel Aluru Postdoctoral Scholar Biology Department Woods Hole Oceanographic Institution Woods Hole, MA 02543 USA 508-289-3607
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Thanks. I think you are right. Thanks to Neel and Jo?o, --Naomi At 03:33 PM 3/11/2011, Neel Aluru wrote: >Hi Naomi, > >I think this error is associated with old >version of edgeR. Try upgrading to the latest >version of edgeR. That's how I was able to get rid of it. > >Neel > >On Mar 11, 2011, at 3:24 PM, Jo?o Moura wrote: > > > Hi Naomi, > > > > just use as.matrix(TotalReads) instead ;) > > > > Cheers > > > > On Fri, Mar 11, 2011 at 8:38 PM, Naomi Altman <naomi at="" stat.psu.edu=""> wrote: > > > >> It looks like calcNormFactors wants a matrix, not a DGEList. Did I do > >> something wrong? > >> > >> d=DGEList(counts=TotalReads, group=treatments,genes=rownames(TotalReads)) > >> > >> Calculating library sizes from column totals. > >> > >> dNorm=calcNormFactors(d) > >> > >> Error in calcNormFactors(d) : 'dataMatrix' needs to be a matrix > >> > >> Naomi > >> > >> _______________________________________________ > >> Bioconductor mailing list > >> Bioconductor at r-project.org > >> https://stat.ethz.ch/mailman/listinfo/bioconductor > >> Search the archives: > >> http://news.gmane.org/gmane.science.biology.informatics.conductor > >> > > > > > > > > -- > > Jo?o Moura > > > > [[alternative HTML version deleted]] > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at r-project.org > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > >Neel Aluru >Postdoctoral Scholar >Biology Department >Woods Hole Oceanographic Institution >Woods Hole, MA 02543 >USA >508-289-3607 > >_______________________________________________ >Bioconductor mailing list >Bioconductor at r-project.org >https://stat.ethz.ch/mailman/listinfo/bioconductor >Search the archives: >http://news.gmane.org/gmane.science.biology.informatics.conductor
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