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Christopher Howerton
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@christopher-howerton-4923
Last seen 7.7 years ago

Hello,
I have been entertaining the notion that high Ct values, which are
generally used as an indicator of no replication due to various
reasons, might actually be a response worth prediction via a binomial
regression. So for example, does a predictor variable (Sex, treatment,
etc.) have a significant effect on the probability of a high or
"undetermined" Ct. Is this sound reasoning? If so, does anyone know of
an implementation to apply a binomial regression to high-throughput
data? (In my case, it would be a miR HTqPCR array).
Thanks for any discussion/suggestions,
Chris
--
Christopher Howerton, PhD
Postdoctoral Researcher
(215) 898-1368
University of Pennsylvania
201E Vet
3800 Spruce Street
Philadelphia, PA 19104-6046