Affy U133 array CEL files - how can i see individual probe signals?
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@guest-user-4897
Last seen 9.6 years ago
Hi Normally,in order to view probe signals from an Affy U133 array i import the CEL using x<-ReadAffy(*.CEL) Then have to y<-rma(x) Which only allows me to see the mean signal for all probes of the same ID, even though they represent different sequences associating with the same target gene. I want to be able to see the signal for every individual probe on the array. Does anyone know of a way of doing this? I have also converted the .CEL file to .txt using Affymetrix Power Tools but it doesn't give me Probe ID names, just x/y co-ords of the probes. Cheers. Deb -- output of sessionInfo(): N/A -- Sent via the guest posting facility at bioconductor.org.
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@sean-davis-490
Last seen 3 months ago
United States
On Wed, Nov 23, 2011 at 7:17 AM, Deb [guest] <guest at="" bioconductor.org=""> wrote: > > Hi > > Normally,in order to view probe signals from an Affy U133 array i import the CEL using > > x<-ReadAffy(*.CEL) Hi, Deb. The object "x" above is of class "AffyBatch". Try taking a look at the help for AffyBatch. Sean > Then have to > > y<-rma(x) > > Which only allows me to see the mean signal for all probes of the same ID, even though they represent different sequences associating with the same target gene. > > I want to be able to see the signal for every individual probe on the array. > > Does anyone know of a way of doing this? > > I have also converted the .CEL file to .txt using Affymetrix Power Tools but it doesn't give me Probe ID names, just x/y co-ords of the probes. > > Cheers. > Deb > > > > ?-- output of sessionInfo(): > > N/A > > -- > Sent via the guest posting facility at bioconductor.org. > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >
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