miRNA array normalization - RMA vs Genespring comparison
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Andrea Grilli ▴ 240
@andrea-grilli-4664
Last seen 8.8 years ago
Italy, Bologna, Rizzoli Orthopaedic Ins…
Dear BioC list, I'm analyzing Agilent miRNa one color arrays in 3 cell lines using AgiMicroRna package (more in detail, it's a time course experiment with 2/3 time points, each in replicate for each cell line, for a total of 16 samples). I normalized with RMA and used linear models as suggested in the manual of the package, getting my "list of d.e. miRs" at each time point. Same data were previously normalized with Genespring and, because of the different normalization, this result has poor overlap with mine. My doubt is not related to the different miRs d.e., but mainly to the fact that in my case I have modest modulations (about 1.5-2.5 Fold change of absolute values), instead in the second case there are changes up to 90 FCs. You can see an image that can clarify the situation at: http://www.mediafire.com/i/?r1s3bir4tqs0fmy Genespring normalization on the left, RMa normalization on the right. With RMA normalization samples show a clear, more uniform, spread, but they are really squeezed on the bottom, so the smaller fold changes. Could be that RMA normalization had "stretch" too much the data? Can I support RMA approach in some way to say that could be "better" than the other using not only this image (or maybe the other is more correct)? Thanks, Andrea
miRNA Normalization GeneSpring miRNA Normalization GeneSpring • 1.0k views
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