Question: flowFlowJo error
gravatar for Gabriel Nathan Kaufman, Mr
7.7 years ago by
When I try to read in a FlowJo (version 7.6.1 for Windows) workspace file into R using the flowFlowJo package, I get a bizarre error that does not seem to be previously described. It appears to be from an underlying R syntax call, but I have not found any reference as to how flowFlowJo generates this message. Is this a problem with my workspace, or a difficulty with the package? I copy the console output below. Note that there is no error traceback available... -------------------------------------------------------- > library(flowFlowJo) Loading required package: MASS Scalable Robust Estimators with High Breakdown Point (version 1.3-01) Warning message: replacing previous import ?.__C__character? when loading ?methods? > FlowJo1 <- readFlowJoList("/Users/kaufman_home/Documents/Mazer lab files/data/Notch-Notch ligand phenotyping/Lung-DCcs-FlowJo.wsp") Getting a workspace for: Lung-DCcs-FlowJo.wsp... got it! Having a bit of trouble finding the FCS files, Will try: /Users/kaufman_home/Documents/Mazer lab files/data/Notch- Notch ligand phenotyping .... found them! Working on: Lung-DCs_Isotype.fcs FSC-A, SSC-A subset:Q1: Comp-APC- Cy7-A- , Comp-PerCP-A+ FSC-A, SSC-A subset:Q2: Comp-APC-Cy7-A+ , Comp-PerCP-A+ FSC-A, SSC-A subset:Q3: Comp-APC-Cy7-A+ , Comp-PerCP-A- FSC-A, SSC-A subset:Q3: Comp-APC-Cy7-A+ , Comp-PerCP-A-:Comp-Alexa Fluor 488-A-Error in gateNodeToFilterObject(gateNode, quiet = TRUE, stripComp = TRUE) : (list) object cannot be coerced to type 'double' > FlowJo1 Error: object 'FlowJo1' not found > traceback() No traceback available > sessionInfo() R version 2.14.1 (2011-12-22) Platform: x86_64-apple-darwin9.8.0/x86_64 (64-bit) locale: [1] en_CA.UTF-8/en_CA.UTF-8/en_CA.UTF-8/C/en_CA.UTF-8/en_CA.UTF-8 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] flowFlowJo_1.12.0 MASS_7.3-16 loaded via a namespace (and not attached): [1] Biobase_2.14.0 feature_1.2.8 flowCore_1.20.0 graph_1.32.0 ks_1.8.5 [6] rrcov_1.3-01 stats4_2.14.1 tools_2.14.1 XML_3.9-2 -------------------------------------------------------- Thank you in advance for your help. Gabriel Kaufman Meakins-Christie Laboratories McGill University Health Centre
flowflowjo • 663 views
ADD COMMENTlink written 7.7 years ago by Gabriel Nathan Kaufman, Mr60
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