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Guest User ★ 12k@guest-user-4897
Last seen 6.6 years ago
hi, I am just a beginner in using R and bioconductor. I have a microarray experiment from the arrayexpress database. http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-109 There are given the target txt and row data (gpr). Now i want used bioconductor to generate a nomalized intensity matrix for all knockout experiments. I thought i have to use limma package and tried to read the data with marray function read.GenePix but there is still an error. Then I tried to read the info txt but there is still an error too. Maybe i just chose the wrong package for my dataset. Unfortunately I don't have a clue how i can generate the normalized datamatrix but the autors of the dataset wrote thez generate the log_fold_change matrix with Imfit limma function. So i thought there is a possibility for me. please help -- output of sessionInfo(): library(limma) library(marray) read.GenePix("3088.gpr") > Error in if (skip > 0L) readLines(file, skip) : > missing value where TRUE/FALSE needed a.target <- read.marrayInfo("E-MTAB-109.sdrf.txt") > Error in names(x) <- value : > 'names' attribute  must be the same length as the vector  -- Sent via the guest posting facility at bioconductor.org.