Question: DEXSeq with no biological replicate?
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gravatar for Sang Chul Choi
7.5 years ago by
Sang Chul Choi230 wrote:
Hi, Is there a way to use DEXSeq with no biological replicate? I used DESeq as explained in the manual to use data with no biological replicate. In the manual of DEXSeq, I could not find a way of using data with no biological replicate. Thank you, SangChul
dexseq • 969 views
ADD COMMENTlink modified 7.5 years ago by Simon Anders3.6k • written 7.5 years ago by Sang Chul Choi230
Answer: DEXSeq with no biological replicate?
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gravatar for Simon Anders
7.5 years ago by
Simon Anders3.6k
Zentrum für Molekularbiologie, Universität Heidelberg
Simon Anders3.6k wrote:
Dear SangChul On 2012-04-02 17:29, Sang Chul Choi wrote: > Is there a way to use DEXSeq with no biological replicate? I used > DESeq as explained in the manual to use data with no biological > replicate. In the manual of DEXSeq, I could not find a way of using > data with no biological replicate. This is intentional. In principle, one could use the same method as we use for DESeq, but this is even more unlikely to give useful results in a test for differential exon usage than for differential expression. The purpose of the "blind" function of DESeq is to give a bioinformatician who is confronted with a data set with a botched experimental design some tool to salvage at least a few minimal results. In my opinion, there is very little point in even trying to perform any kind of more advanced analyses, like testing for differential exon usage, in such a situation. Simon
ADD COMMENTlink written 7.5 years ago by Simon Anders3.6k
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