Fwd: heatmap.2 question
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Alyaa Mahmoud ▴ 440
@alyaa-mahmoud-4670
Last seen 4.1 years ago
Hi All How can I possibly extract the clusters by rows from a heatmap generated by heatmap.2 function. i.e. the heatmap is generated with colouring for every cluster of rows and I want to retrieve the members of each cluster. Any clue would be much appreciated Thank you very much Alyaa KAUST/AUC Marine Genomics Project American University in Cairo Egypt 2918 -- Alyaa Mahmoud "Love all, trust a few, do wrong to none"- Shakespeare -- Alyaa Mahmoud "Love all, trust a few, do wrong to none"- Shakespeare [[alternative HTML version deleted]]
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Wang, Li ▴ 180
@wang-li-5216
Last seen 9.6 years ago
Hi, Alyaa Assuming that you have an object "hr" which clusters rows by spearman correlation as distance method, hr <- hclust(as.dist(1-cor(t(y), method="spearman")), method="complete"), y is your matrix here. hr$labels[hr$order] it will print the row labels in the order they appear in the tree. I donot know if this is what you want. Best Li ________________________________________ From: bioconductor-bounces@r-project.org [bioconductor- bounces@r-project.org] On Behalf Of Alyaa Mahmoud [alyamahmoud@gmail.com] Sent: Thursday, May 24, 2012 10:04 AM To: bioconductor at r-project.org Subject: [BioC] Fwd: heatmap.2 question Hi All How can I possibly extract the clusters by rows from a heatmap generated by heatmap.2 function. i.e. the heatmap is generated with colouring for every cluster of rows and I want to retrieve the members of each cluster. Any clue would be much appreciated Thank you very much Alyaa KAUST/AUC Marine Genomics Project American University in Cairo Egypt 2918 -- Alyaa Mahmoud "Love all, trust a few, do wrong to none"- Shakespeare -- Alyaa Mahmoud "Love all, trust a few, do wrong to none"- Shakespeare [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list Bioconductor at r-project.org https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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@james-w-macdonald-5106
Last seen 16 hours ago
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Hi Alyaa, On 5/24/2012 11:04 AM, Alyaa Mahmoud wrote: > Hi All > > How can I possibly extract the clusters by rows from a heatmap generated by > heatmap.2 function. i.e. the heatmap is generated with colouring for every > cluster of rows and I want to retrieve the members of each cluster. Note that heatmap.2() uses hclust() to create the clusters. There is a function cutree() that can be used to get the groups from the output of hclust(). Also note that heatmap.2() invisibly returns a list that includes both the row and column dendrograms (by returning the list invisibly, you don't get a bunch of junk printed out to your console. However, if you use the assignment function (<-) to assign the output to an object, you can then extract whichever dendrogram you want.). Best, Jim > > > Any clue would be much appreciated > > Thank you very much > Alyaa > > KAUST/AUC Marine Genomics Project > American University in Cairo > Egypt > 2918 > -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099
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Also: Someone correct me if I'm wrong, as I often turn myself around on this one, but also note that the y-axis you see on the heatmap.2 is "flipped." So the "first" row (`result$rowInd[1]`) is at the bottom of the heatmap, not the top. -steve On Thu, May 24, 2012 at 11:18 AM, James W. MacDonald <jmacdon at="" uw.edu=""> wrote: > Hi Alyaa, > > > On 5/24/2012 11:04 AM, Alyaa Mahmoud wrote: >> >> Hi All >> >> How can I possibly extract the clusters by rows from a heatmap generated >> by >> heatmap.2 function. i.e. the heatmap is generated with colouring for every >> cluster of rows and I want to retrieve the members of each cluster. > > > Note that heatmap.2() uses hclust() to create the clusters. There is a > function cutree() that can be used to get the groups from the output of > hclust(). > > Also note that heatmap.2() invisibly returns a list that includes both the > row and column dendrograms (by returning the list invisibly, you don't get a > bunch of junk printed out to your console. However, if you use the > assignment function (<-) to assign the output to an object, you can then > extract whichever dendrogram you want.). > > Best, > > Jim > > > >> >> >> Any clue would be much appreciated >> >> Thank you very much >> Alyaa >> >> KAUST/AUC Marine Genomics Project >> American University in Cairo >> Egypt >> 2918 >> > > -- > James W. MacDonald, M.S. > Biostatistician > University of Washington > Environmental and Occupational Health Sciences > 4225 Roosevelt Way NE, # 100 > Seattle WA 98105-6099 > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor -- Steve Lianoglou Graduate Student: Computational Systems Biology ?| Memorial Sloan-Kettering Cancer Center ?| Weill Medical College of Cornell University Contact Info: http://cbio.mskcc.org/~lianos/contact
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Yep. The heatmap itself is created using image(), which plots the matrix from the bottom up, so the [1,1] position of the matrix is at the lower left of the image. Best, Jim On 5/24/2012 11:29 AM, Steve Lianoglou wrote: > Also: Someone correct me if I'm wrong, as I often turn myself around > on this one, but also note that the y-axis you see on the heatmap.2 is > "flipped." So the "first" row (`result$rowInd[1]`) is at the bottom of > the heatmap, not the top. > > -steve > > On Thu, May 24, 2012 at 11:18 AM, James W. MacDonald<jmacdon at="" uw.edu=""> wrote: >> Hi Alyaa, >> >> >> On 5/24/2012 11:04 AM, Alyaa Mahmoud wrote: >>> Hi All >>> >>> How can I possibly extract the clusters by rows from a heatmap generated >>> by >>> heatmap.2 function. i.e. the heatmap is generated with colouring for every >>> cluster of rows and I want to retrieve the members of each cluster. >> >> Note that heatmap.2() uses hclust() to create the clusters. There is a >> function cutree() that can be used to get the groups from the output of >> hclust(). >> >> Also note that heatmap.2() invisibly returns a list that includes both the >> row and column dendrograms (by returning the list invisibly, you don't get a >> bunch of junk printed out to your console. However, if you use the >> assignment function (<-) to assign the output to an object, you can then >> extract whichever dendrogram you want.). >> >> Best, >> >> Jim >> >> >> >>> >>> Any clue would be much appreciated >>> >>> Thank you very much >>> Alyaa >>> >>> KAUST/AUC Marine Genomics Project >>> American University in Cairo >>> Egypt >>> 2918 >>> >> -- >> James W. MacDonald, M.S. >> Biostatistician >> University of Washington >> Environmental and Occupational Health Sciences >> 4225 Roosevelt Way NE, # 100 >> Seattle WA 98105-6099 >> >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor > > -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099
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Thanks to all of you, this was really helpful :) Alyaa On Thu, May 24, 2012 at 6:42 PM, James W. MacDonald <jmacdon@uw.edu> wrote: > Yep. The heatmap itself is created using image(), which plots the matrix > from the bottom up, so the [1,1] position of the matrix is at the lower > left of the image. > > > Best, > > Jim > > > On 5/24/2012 11:29 AM, Steve Lianoglou wrote: > >> Also: Someone correct me if I'm wrong, as I often turn myself around >> on this one, but also note that the y-axis you see on the heatmap.2 is >> "flipped." So the "first" row (`result$rowInd[1]`) is at the bottom of >> the heatmap, not the top. >> >> -steve >> >> On Thu, May 24, 2012 at 11:18 AM, James W. MacDonald<jmacdon@uw.edu> >> wrote: >> >>> Hi Alyaa, >>> >>> >>> On 5/24/2012 11:04 AM, Alyaa Mahmoud wrote: >>> >>>> Hi All >>>> >>>> How can I possibly extract the clusters by rows from a heatmap generated >>>> by >>>> heatmap.2 function. i.e. the heatmap is generated with colouring for >>>> every >>>> cluster of rows and I want to retrieve the members of each cluster. >>>> >>> >>> Note that heatmap.2() uses hclust() to create the clusters. There is a >>> function cutree() that can be used to get the groups from the output of >>> hclust(). >>> >>> Also note that heatmap.2() invisibly returns a list that includes both >>> the >>> row and column dendrograms (by returning the list invisibly, you don't >>> get a >>> bunch of junk printed out to your console. However, if you use the >>> assignment function (<-) to assign the output to an object, you can then >>> extract whichever dendrogram you want.). >>> >>> Best, >>> >>> Jim >>> >>> >>> >>> >>>> Any clue would be much appreciated >>>> >>>> Thank you very much >>>> Alyaa >>>> >>>> KAUST/AUC Marine Genomics Project >>>> American University in Cairo >>>> Egypt >>>> 2918 >>>> >>>> -- >>> James W. MacDonald, M.S. >>> Biostatistician >>> University of Washington >>> Environmental and Occupational Health Sciences >>> 4225 Roosevelt Way NE, # 100 >>> Seattle WA 98105-6099 >>> >>> >>> ______________________________**_________________ >>> Bioconductor mailing list >>> Bioconductor@r-project.org >>> https://stat.ethz.ch/mailman/**listinfo/bioconductor<https: stat.="" ethz.ch="" mailman="" listinfo="" bioconductor=""> >>> Search the archives: >>> http://news.gmane.org/gmane.**science.biology.informatics.**conduc tor<http: news.gmane.org="" gmane.science.biology.informatics.conductor=""> >>> >> >> >> > -- > James W. MacDonald, M.S. > Biostatistician > University of Washington > Environmental and Occupational Health Sciences > 4225 Roosevelt Way NE, # 100 > Seattle WA 98105-6099 > > -- Alyaa Mahmoud "Love all, trust a few, do wrong to none"- Shakespeare [[alternative HTML version deleted]]
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