Wheat annotation
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Hello, I am working on a set of Affymetrix wheat array, with some very ancient annotations included. I have been looking, and many sequenced organisms have a xxx.db annotation package that is used in conjunction with annotationDBi. I have found the wheatcdf, which seems to describe the environment (probe mapping), but this does not seem to be the same information as the xxx.db has. Is there such a package, or is wheat without such annotations? Thanks! Sam -- output of sessionInfo(): = -- Sent via the guest posting facility at bioconductor.org.
Annotation cdf AnnotationDbi Annotation cdf AnnotationDbi • 1.3k views
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@james-w-macdonald-5106
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Hi Sam, On 6/12/2012 8:58 PM, Sam McInturf [guest] wrote: > Hello, > I am working on a set of Affymetrix wheat array, with some very ancient annotations included. I have been looking, and many sequenced organisms have a xxx.db annotation package that is used in conjunction with annotationDBi. I have found the wheatcdf, which seems to describe the environment (probe mapping), but this does not seem to be the same information as the xxx.db has. Is there such a package, or is wheat without such annotations? There is no such package. These packages depend on some fairly extensive infrastructure (they need an organism-level .db package, as well as an intermediate 'db0' package that Marc Carlson makes). Which packages have been supported is based on the cost/benefit where benefit is defined loosely as the number of end users who are likely to use the package. Unfortunately anopheles and yeast are the only plants to have jumped that hurdle so far. I checked the biomaRt package as well, and I don't see any support for Triticum there either. You could always just do something bootleg like downloading the annotations from Affy http://www.affymetrix.com/analysis/downloads/na32/ivt/wheat.na32.annot .csv.zip and parsing by hand. Best, Jim > > Thanks! > Sam > > -- output of sessionInfo(): > > = > > -- > Sent via the guest posting facility at bioconductor.org. > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099
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Hello, On Wed, Jun 13, 2012 at 10:07 AM, James W. MacDonald <jmacdon at="" uw.edu=""> wrote: > Unfortunately > anopheles and yeast are the only plants to have jumped that hurdle so far. Neither of these is a plant, though :) Take care Oliver -- Oliver Ruebenacker Bioinformatics Consultant (http://www.knowomics.com/wiki/Oliver_Ruebenacker) Knowomics, The Bioinformatics Network (http://www.knowomics.com) SBPAX: Turning Bio Knowledge into Math Models (http://www.sbpax.org)
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On 6/13/2012 12:09 PM, Oliver Ruebenacker wrote: > Hello, > > On Wed, Jun 13, 2012 at 10:07 AM, James W. MacDonald<jmacdon at="" uw.edu=""> wrote: >> Unfortunately >> anopheles and yeast are the only plants to have jumped that hurdle so far. > Neither of these is a plant, though :) So you say. Next time an Anopheles *plants* its proboscis in your arm, give me a call. ;-D > > Take care > Oliver > -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099
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The affymetrix annotations are sparse at best, I think I will have to blastp to establish a file that I can use to annotate the genes using custom functions. Thank you for confirming my observation that the wheat annotations are not so available. Sam On Wed, Jun 13, 2012 at 11:16 AM, James W. MacDonald <jmacdon@uw.edu> wrote: > > > On 6/13/2012 12:09 PM, Oliver Ruebenacker wrote: > >> Hello, >> >> On Wed, Jun 13, 2012 at 10:07 AM, James W. MacDonald<jmacdon@uw.edu> >> wrote: >> >>> Unfortunately >>> anopheles and yeast are the only plants to have jumped that hurdle so >>> far. >>> >> Neither of these is a plant, though :) >> > > So you say. Next time an Anopheles *plants* its proboscis in your arm, > give me a call. ;-D > > >> Take care >> Oliver >> >> > -- > James W. MacDonald, M.S. > Biostatistician > University of Washington > Environmental and Occupational Health Sciences > 4225 Roosevelt Way NE, # 100 > Seattle WA 98105-6099 > > -- Sam McInturf [[alternative HTML version deleted]]
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Hi Sam, The only plant we have very thorough support for (so far) is arabidopsis. But if you need an organism package for wheat, you can try to generate one using the makeOrgPackageFromNCBI() function from the AnnotationDbi package. To do that you will need to specify the tax ID you want though. So (as an example), is tax ID //4565 the kind of wheat that you are looking for (link below)??? http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=4 565&lvl=3&lin=f&keep=1&srchmode=1&unlock Please let me know if you still have questions, Marc On 06/13/2012 11:39 AM, Sam McInturf wrote: > The affymetrix annotations are sparse at best, I think I will have to > blastp to establish a file that I can use to annotate the genes using > custom functions. > Thank you for confirming my observation that the wheat annotations are not > so available. > > Sam > > On Wed, Jun 13, 2012 at 11:16 AM, James W. MacDonald<jmacdon@uw.edu> wrote: > >> >> On 6/13/2012 12:09 PM, Oliver Ruebenacker wrote: >> >>> Hello, >>> >>> On Wed, Jun 13, 2012 at 10:07 AM, James W. MacDonald<jmacdon@uw.edu> >>> wrote: >>> >>>> Unfortunately >>>> anopheles and yeast are the only plants to have jumped that hurdle so >>>> far. >>>> >>> Neither of these is a plant, though :) >>> >> So you say. Next time an Anopheles *plants* its proboscis in your arm, >> give me a call. ;-D >> >> >>> Take care >>> Oliver >>> >>> >> -- >> James W. MacDonald, M.S. >> Biostatistician >> University of Washington >> Environmental and Occupational Health Sciences >> 4225 Roosevelt Way NE, # 100 >> Seattle WA 98105-6099 >> >> > [[alternative HTML version deleted]]
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