I'm performing some microarrays analysis for a simple case(15 microarrays), control(3 microarrays) experiment design. Don't ask me the reason for which i have a so unbalanced dataset ;-)
In order to detect differentially expressed genes I wish to perform a LIMMA analysis...but checking the omogeneity of variances with bartlett test I observ a difference statistically significative between cases and controls.
According to your experience: Is a good idea before doing a parametric analysis checking the variances
utilizing Bartlett test? In my case a non parametric test(like SAM) might be better than LIMMA?
thak you for any tips