I need to split a big SFF file into a number of small SFF files. I was able to read in the big SFF file as a big SFFContainer with the function readSFF in the Bioconductor package:R453Plus1Toolbox. I also fond the index to split the big SFFContainer. For example, the index (5,11,17,22,25,29,31,33,37) for the first small SFFContainer. I couldn't figure out how to extract the (5,11,17,22,25,29,31,33,37)th reads from the big SSFContainer and construct a small SFFContainer and save it as an SFF file. Could you please tell me how to do this part of work? Thank you very much in advance. Heidi -- output of sessionInfo(): > sessionInfo() R version 2.15.1 (2012-06-22) Platform: x86_64-pc-mingw32/x64 (64-bit) locale: [1] LC_COLLATE=English_United States.1252 [2] LC_CTYPE=English_United States.1252 [3] LC_MONETARY=English_United States.1252 [4] LC_NUMERIC=C [5] LC_TIME=English_United States.1252 attached base packages: [1] stats graphics grDevices utils datasets methods base -- Sent via the guest posting facility at bioconductor.org.