HT qPCR; Creating qPCRset from expression matrix
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@ali-mohammadian-5008
Last seen 7.2 years ago
Hi all! How is it possible to create a qPCRset object from an expression matrix (in new veersions of HTqCR?) Thank you indeed very much.
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Heidi Dvinge ★ 2.0k
@heidi-dvinge-2195
Last seen 7.2 years ago
> Hi all! > > How is it possible to create a qPCRset object from an expression > matrix (in new veersions of HTqCR?) > Basically the same way as before ;) There's an example is the vignette, but in the (admittedly not very informative titled) "Additional devices" section. E.g. mat <- matrix(rnorm(9*96), ncol = 6, nrow = 96, byrow = FALSE) raw <- new("qPCRset", exprs = mat, featureCategory = as.data.frame(array("OK", dim=dim(mat)))) sampleNames(raw) <- paste("S", 1:6, sep = "") featureNames(raw) <- paste("A", 1:96, sep = "") This is the simplest approach. Alternatively, if you e.g. want more information about your samples, you can add phenoData to the object either during or after creating it. See ?'qPCRset-class' for more details about the qPCRset objects. HTH \Heidi > Thank you indeed very much. > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor >
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