Dear List, I am having difficulty reading in multiple text files using the 'readQpcrBatch' function from qpcrNorm. Reading in single files is not a problem: > data=readQpcr("file1.txt",header=FALSE) > slotNames(data) [1] "geneNames" "plateIndex" "exprs" "normalized" "normGenes" # However, trying to read all text files from an autonomous folder results in this error: > data=readQpcrBatch() Error in initialize(value, ...) : no slot of name "primerNames" for this object of class "qpcrBatch" #The columns conform to the requirements of readQpcr and I get this error even if I read in the data file 'qpcrBatch.object ' that is provided with the vignette as a text file. #The same outcome results from: > data=readQpcrBatch(filenames=c("file1.txt","file2.txt"),header=FALSE) Error in initialize(value, ...) : no slot of name "primerNames" for this object of class "qpcrBatch" > sessionInfo() R version 2.15.1 (2012-06-22) Platform: x86_64-pc-mingw32/x64 (64-bit) locale: [1] LC_COLLATE=English_United Kingdom.1252 [2] LC_CTYPE=English_United Kingdom.1252 [3] LC_MONETARY=English_United Kingdom.1252 [4] LC_NUMERIC=C [5] LC_TIME=English_United Kingdom.1252 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] qpcrNorm_1.14.0 affy_1.34.0 limma_3.12.1 Biobase_2.16.0 [5] BiocGenerics_0.2.0 loaded via a namespace (and not attached): [1] affyio_1.24.0 BiocInstaller_1.4.7 preprocessCore_1.18.0 [4] tools_2.15.1 zlibbioc_1.2.0 Any suggestions are gratefully received. Regards Alan Alan James Mueller BSc BVM&S MRes MRCVS Room 1.02 Comparative Musculoskeletal Research Group The School of Veterinary Science University of Liverpool Leahurst Chester High Road Neston CH64 7TE UK