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@guest-user-4897
Last seen 9.6 years ago
Dear all,
I am having trouble reading in some FCS files into a flow core session
using the flowSet function.
When I try to read the files in individually using the read.FCS
command I have no problems.
Here's my code:
files.pico<-list.files('~/Documents/Flow Cytometry/Picoplankton',
all.files=TRUE, full.names=TRUE)
set.pico<-read.flowSet(files.pico)
This gives he follow error message:
Error in if (!version %in% c("FCS2.0", "FCS3.0")) stop("This does not
seem to be a valid FCS2.0 or FCS3.0 file") :
argument is of length zero
However, when I load in one of the files using read.FCS it works fine,
which suggests that my files which have come from BD cell quest pro
are indeed valid.
p1<-read.FCS('~/Documents/Flow Cytometry/Picoplankton/01_P01.124',
transform='scale', alter.names=TRUE)
Any ideas as to what might be causing the problem?
Thanks in advance.
-- output of sessionInfo():
R version 2.15.1 (2012-06-22)
Platform: i386-apple-darwin9.8.0/i386 (32-bit)
locale:
[1] en_GB.UTF-8/en_GB.UTF-8/en_GB.UTF-8/C/en_GB.UTF-8/en_GB.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] flowViz_1.20.0 lattice_0.20-6 flowCore_1.22.3
rrcov_1.3-02
[5] pcaPP_1.9-47 mvtnorm_0.9-9992 robustbase_0.9-3
Biobase_2.16.0
[9] BiocGenerics_0.2.0
loaded via a namespace (and not attached):
[1] feature_1.2.8 graph_1.34.0 grid_2.15.1
KernSmooth_2.23-7
[5] ks_1.8.8 latticeExtra_0.6-24 MASS_7.3-18
RColorBrewer_1.0-5
[9] stats4_2.15.1 tools_2.15.1
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