Question: Is normalization in edgeR required for small RNA sequencing data?
7.2 years ago by
Danie • 40
Danie • 40 wrote:
Dear All, I am PhD student, currently working on differential expression analysis of my smallRNA library deep sequencing data and trying to identify differentially expressed miRNAs, using edgeR package. I have 24 different samples with 2 biological replicates (48 libraries). I am performing multiple group comparison using GLM method and also Anova-like test to idetify DE miRNAs among the different groups of my samples. My question is : Do I need to normalize my input data using *calcNormFactors() *once I set my DGE list or I could proceed without any normalization? I assume in this case that edgeR performs a default normallization when it is "calculating library sizes from column totals"? I would really appreciate any suggestion on this! Thanks in advance, Daniela [[alternative HTML version deleted]]
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