Question: Expression on different Affy arrays
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gravatar for donghu@itsa.ucsf.edu
15.2 years ago by
donghu@itsa.ucsf.edu70 wrote:
Hi, I have a few MOE430A and MOE430 2.0 arrays. As you know, MOE430A has ~22000 probe sets and MOE430 2.0 has ~46000 probe sets. All MOE430A probe sets are on MOE430 2.0. Now I need to compare expression measurement of same probe sets on these 2 types of array. Is it possible to compute RMA measurement of these 2 array types? If there is no convenient way to do so, does it sound reasonable to use MAS 5.0 signal intensity for such comparison as MAS 5.0 uses a target intensity for normalization? Thanks for any suggestions. Regards, Donglei Hu
moe430a probe • 457 views
ADD COMMENTlink modified 15.2 years ago by Park, Richard220 • written 15.2 years ago by donghu@itsa.ucsf.edu70
Answer: Expression on different Affy arrays
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gravatar for Park, Richard
15.2 years ago by
Park, Richard220
Park, Richard220 wrote:
Hi Donglei, I think using MAS 5 is a perfectly reasonable way to go w/ out being able to use rma to a complete background normalization. I would just be careful about trying to deduce signifigance from genes w/ lower expression and lower fold change values. This kind of analysis would need to be broad. The only possible way of combining the 2 different chips into one rma normalization to my knowledge. Would be trying to subset the 2.0 arrays into a format for the 1.0 version. This would require understanding the .cel format and then downloading the individual probeset information (pm and mm values for each x by y pair). It's not an easy task, but it is possible. And also you may not know what can increase the noise into the chips (different number of amplifications, different starting amounts of rna, chip defects, etc.. etc..) hth, richard -----Original Message----- From: donghu@itsa.ucsf.edu [mailto:donghu@itsa.ucsf.edu] Sent: Wednesday, June 16, 2004 5:53 PM To: bioconductor@stat.math.ethz.ch Subject: [BioC] Expression on different Affy arrays Hi, I have a few MOE430A and MOE430 2.0 arrays. As you know, MOE430A has ~22000 probe sets and MOE430 2.0 has ~46000 probe sets. All MOE430A probe sets are on MOE430 2.0. Now I need to compare expression measurement of same probe sets on these 2 types of array. Is it possible to compute RMA measurement of these 2 array types? If there is no convenient way to do so, does it sound reasonable to use MAS 5.0 signal intensity for such comparison as MAS 5.0 uses a target intensity for normalization? Thanks for any suggestions. Regards, Donglei Hu _______________________________________________ Bioconductor mailing list Bioconductor@stat.math.ethz.ch https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor
ADD COMMENTlink written 15.2 years ago by Park, Richard220
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