[methylumi] concerning methylumi.bgcorr function and out-of-band intensities
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@hannah-elliott-5893
Last seen 9.6 years ago
Dylan A?ssi <dylan.aissi at="" ...=""> writes: > > Dear all, > I would like to understand what really does the methylumi.bgcorr function > with method='noob' but I can't find information about out-of-band > intensities. > > Does anyone know what is meant by out-of-band intensities? > > Thank you. > > Dylan A?ssi > > PhD student > INSERM UMRS 937 > Facult? de M?decine Pierre et Marie Curie > 91 Boulevard de l'H?pital > 75013 Paris > > [[alternative HTML version deleted]] > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at ... > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor Dear Dylan, The use of out of band intensities for background correction is described in detail in this paper: http://www.ncbi.nlm.nih.gov/pubmed/23476028. Signal intensity is measured from type I green probes in the red channel & type I red probes in the green channel as an alternative method of deriving the background signal from the array. I hope this helps answer your question. Hannah Elliott University of Bristol
methylumi methylumi • 851 views
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